Title |
The Constructor: a web application optimizing cloning strategies based on modules from the registry of standard biological parts
|
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Published in |
Journal of Biological Engineering, December 2012
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DOI | 10.1186/1754-1611-6-14 |
Pubmed ID | |
Authors |
Matthijn C Hesselman, Jasper J Koehorst, Thijs Slijkhuis, Dorett I Odoni, Floor Hugenholtz, MarkW J van Passel |
Abstract |
Synthetic biology is an emerging field that combines molecular biology with engineering principles, which requires abstraction levels applied to a modular biological componentry. The Registry of Standard Biological Parts harbours such a repository of standardized parts, and thereby facilitates the combination of complex molecular modules to novel genetic circuits and devices. However, since finding the best parts for a pre-determined genetic design can be time consuming, we devised the Constructor, a web tool that recommends the smallest number of cloning steps for pre-designed circuits, and implements user-defined quality checks.We present the Constructor ( http://www.systemsbiology.nl/the_constructor) as a constructive web tool that simplifies the in silico assembly of pre-designed gene circuitries from standard parts, reducing both planning and subsequent cloning time. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 2 | 7% |
Spain | 1 | 4% |
United Kingdom | 1 | 4% |
Unknown | 23 | 85% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 10 | 37% |
Student > Ph. D. Student | 8 | 30% |
Student > Master | 3 | 11% |
Other | 2 | 7% |
Student > Bachelor | 1 | 4% |
Other | 2 | 7% |
Unknown | 1 | 4% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 14 | 52% |
Biochemistry, Genetics and Molecular Biology | 6 | 22% |
Social Sciences | 2 | 7% |
Immunology and Microbiology | 1 | 4% |
Nursing and Health Professions | 1 | 4% |
Other | 0 | 0% |
Unknown | 3 | 11% |