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MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments

Overview of attention for article published in BMC Molecular Biology, January 2010
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • One of the highest-scoring outputs from this source (#5 of 302)
  • High Attention Score compared to outputs of the same age (94th percentile)

Mentioned by

blogs
3 blogs
wikipedia
1 Wikipedia page

Citations

dimensions_citation
498 Dimensions

Readers on

mendeley
856 Mendeley
citeulike
5 CiteULike
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Title
MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments
Published in
BMC Molecular Biology, January 2010
DOI 10.1186/1471-2199-11-74
Pubmed ID
Authors

Stephen A Bustin, Jean-François Beaulieu, Jim Huggett, Rolf Jaggi, Frederick SB Kibenge, Pål A Olsvik, Louis C Penning, Stefan Toegel

Abstract

The conclusions of thousands of peer-reviewed publications rely on data obtained using fluorescence-based quantitative real-time PCR technology. However, the inadequate reporting of experimental detail, combined with the frequent use of flawed protocols is leading to the publication of papers that may not be technically appropriate. We take the view that this problem requires the delineation of a more transparent and comprehensive reporting policy from scientific journals. This editorial aims to provide practical guidance for the incorporation of absolute minimum standards encompassing the key assay parameters for accurate design, documentation and reporting of qPCR experiments (MIQE précis) and guidance on the publication of pure 'reference gene' articles.

Mendeley readers

The data shown below were compiled from readership statistics for 856 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 7 <1%
Mexico 6 <1%
Brazil 6 <1%
Germany 5 <1%
France 4 <1%
United Kingdom 4 <1%
Spain 3 <1%
South Africa 3 <1%
Chile 2 <1%
Other 18 2%
Unknown 798 93%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 221 26%
Researcher 180 21%
Student > Master 120 14%
Student > Bachelor 53 6%
Student > Doctoral Student 47 5%
Other 167 20%
Unknown 68 8%
Readers by discipline Count As %
Agricultural and Biological Sciences 471 55%
Biochemistry, Genetics and Molecular Biology 118 14%
Medicine and Dentistry 57 7%
Environmental Science 21 2%
Engineering 16 2%
Other 81 9%
Unknown 92 11%

Attention Score in Context

This research output has an Altmetric Attention Score of 20. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 June 2019.
All research outputs
#1,352,691
of 20,278,033 outputs
Outputs from BMC Molecular Biology
#5
of 302 outputs
Outputs of similar age
#8,533
of 146,593 outputs
Outputs of similar age from BMC Molecular Biology
#1
of 1 outputs
Altmetric has tracked 20,278,033 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 93rd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 302 research outputs from this source. They receive a mean Attention Score of 3.1. This one has done particularly well, scoring higher than 98% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 146,593 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 94% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them