Title |
High- and low-affinity cre boxes for CcpA binding in Bacillus subtilis revealed by genome-wide analysis
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Published in |
BMC Genomics, August 2012
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DOI | 10.1186/1471-2164-13-401 |
Pubmed ID | |
Authors |
Bogumiła C Marciniak, Monika Pabijaniak, Anne de Jong, Robert Dűhring, Gerald Seidel, Wolfgang Hillen, Oscar P Kuipers |
Abstract |
In Bacillus subtilis and its relatives carbon catabolite control, a mechanism enabling to reach maximal efficiency of carbon and energy sources metabolism, is achieved by the global regulator CcpA (carbon catabolite protein A). CcpA in a complex with HPr-Ser-P (seryl-phosphorylated form of histidine-containing protein, HPr) binds to operator sites called catabolite responsive elements, cre. Depending on the cre box position relative to the promoter, the CcpA/HPr-Ser-P complex can either act as a positive or a negative regulator. The cre boxes are highly degenerate semi-palindromes with a lowly conserved consensus sequence. So far, studies aimed at revealing how CcpA can bind such diverse sites were focused on the analysis of single cre boxes. In this study, a genome-wide analysis of cre sites was performed in order to identify differences in cre sequence and position, which determine their binding affinity. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Netherlands | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 1% |
Netherlands | 1 | 1% |
Unknown | 82 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 30 | 36% |
Researcher | 11 | 13% |
Student > Bachelor | 8 | 10% |
Student > Master | 6 | 7% |
Professor > Associate Professor | 5 | 6% |
Other | 13 | 15% |
Unknown | 11 | 13% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 34 | 40% |
Agricultural and Biological Sciences | 31 | 37% |
Immunology and Microbiology | 6 | 7% |
Unknown | 13 | 15% |