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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Direct Detection and Sequencing of Damaged DNA Bases
|
|---|---|
| Published in |
Genome Integrity, December 2011
|
| DOI | 10.1186/2041-9414-2-10 |
| Pubmed ID | |
| Authors |
Tyson A Clark, Kristi E Spittle, Stephen W Turner, Jonas Korlach |
| Abstract |
Products of various forms of DNA damage have been implicated in a variety of important biological processes, such as aging, neurodegenerative diseases, and cancer. Therefore, there exists great interest to develop methods for interrogating damaged DNA in the context of sequencing. Here, we demonstrate that single-molecule, real-time (SMRT®) DNA sequencing can directly detect damaged DNA bases in the DNA template - as a by-product of the sequencing method - through an analysis of the DNA polymerase kinetics that are altered by the presence of a modified base. We demonstrate the sequencing of several DNA templates containing products of DNA damage, including 8-oxoguanine, 8-oxoadenine, O6-methylguanine, 1-methyladenine, O4-methylthymine, 5-hydroxycytosine, 5-hydroxyuracil, 5-hydroxymethyluracil, or thymine dimers, and show that these base modifications can be readily detected with single-modification resolution and DNA strand specificity. We characterize the distinct kinetic signatures generated by these DNA base modifications. |
X Demographics
The data shown below were collected from the profiles of 6 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 33% |
| United Kingdom | 2 | 33% |
| Cameroon | 1 | 17% |
| Unknown | 1 | 17% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 4 | 67% |
| Practitioners (doctors, other healthcare professionals) | 1 | 17% |
| Members of the public | 1 | 17% |
Mendeley readers
The data shown below were compiled from readership statistics for 174 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 7 | 4% |
| Netherlands | 1 | <1% |
| India | 1 | <1% |
| United Kingdom | 1 | <1% |
| Argentina | 1 | <1% |
| France | 1 | <1% |
| China | 1 | <1% |
| Belgium | 1 | <1% |
| Japan | 1 | <1% |
| Other | 1 | <1% |
| Unknown | 158 | 91% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 47 | 27% |
| Student > Ph. D. Student | 35 | 20% |
| Student > Master | 17 | 10% |
| Student > Doctoral Student | 12 | 7% |
| Student > Bachelor | 10 | 6% |
| Other | 33 | 19% |
| Unknown | 20 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 78 | 45% |
| Biochemistry, Genetics and Molecular Biology | 48 | 28% |
| Chemistry | 6 | 3% |
| Computer Science | 6 | 3% |
| Engineering | 5 | 3% |
| Other | 12 | 7% |
| Unknown | 19 | 11% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 February 2023.
All research outputs
#4,835,823
of 25,373,627 outputs
Outputs from Genome Integrity
#12
of 38 outputs
Outputs of similar age
#37,622
of 248,898 outputs
Outputs of similar age from Genome Integrity
#1
of 1 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. Compared to these this one has done well and is in the 79th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 38 research outputs from this source. They receive a mean Attention Score of 3.9. This one scored the same or higher as 26 of them.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 248,898 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 83% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them