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Direct Detection and Sequencing of Damaged DNA Bases

Overview of attention for article published in Genome Integrity, January 2011
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (78th percentile)

Mentioned by

twitter
5 tweeters
wikipedia
2 Wikipedia pages

Citations

dimensions_citation
64 Dimensions

Readers on

mendeley
159 Mendeley
citeulike
2 CiteULike
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Title
Direct Detection and Sequencing of Damaged DNA Bases
Published in
Genome Integrity, January 2011
DOI 10.1186/2041-9414-2-10
Pubmed ID
Authors

Tyson A Clark, Kristi E Spittle, Stephen W Turner, Jonas Korlach

Abstract

Products of various forms of DNA damage have been implicated in a variety of important biological processes, such as aging, neurodegenerative diseases, and cancer. Therefore, there exists great interest to develop methods for interrogating damaged DNA in the context of sequencing. Here, we demonstrate that single-molecule, real-time (SMRT®) DNA sequencing can directly detect damaged DNA bases in the DNA template - as a by-product of the sequencing method - through an analysis of the DNA polymerase kinetics that are altered by the presence of a modified base. We demonstrate the sequencing of several DNA templates containing products of DNA damage, including 8-oxoguanine, 8-oxoadenine, O6-methylguanine, 1-methyladenine, O4-methylthymine, 5-hydroxycytosine, 5-hydroxyuracil, 5-hydroxymethyluracil, or thymine dimers, and show that these base modifications can be readily detected with single-modification resolution and DNA strand specificity. We characterize the distinct kinetic signatures generated by these DNA base modifications.

Twitter Demographics

The data shown below were collected from the profiles of 5 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 159 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 7 4%
Argentina 1 <1%
India 1 <1%
United Kingdom 1 <1%
France 1 <1%
Belgium 1 <1%
China 1 <1%
Japan 1 <1%
Netherlands 1 <1%
Other 1 <1%
Unknown 143 90%

Demographic breakdown

Readers by professional status Count As %
Researcher 45 28%
Student > Ph. D. Student 33 21%
Student > Master 16 10%
Student > Doctoral Student 11 7%
Student > Bachelor 10 6%
Other 32 20%
Unknown 12 8%
Readers by discipline Count As %
Agricultural and Biological Sciences 77 48%
Biochemistry, Genetics and Molecular Biology 46 29%
Chemistry 7 4%
Computer Science 5 3%
Engineering 4 3%
Other 9 6%
Unknown 11 7%

Attention Score in Context

This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 January 2020.
All research outputs
#3,291,029
of 16,547,829 outputs
Outputs from Genome Integrity
#6
of 16 outputs
Outputs of similar age
#25,984
of 137,550 outputs
Outputs of similar age from Genome Integrity
#1
of 1 outputs
Altmetric has tracked 16,547,829 research outputs across all sources so far. Compared to these this one has done well and is in the 76th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 16 research outputs from this source. They receive a mean Attention Score of 3.5. This one scored the same or higher as 10 of them.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 137,550 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 78% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them