Title |
The LO-BaFL method and ALS microarray expression analysis
|
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Published in |
BMC Bioinformatics, September 2012
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DOI | 10.1186/1471-2105-13-244 |
Pubmed ID | |
Authors |
Cristina Baciu, Kevin J Thompson, Jean-Luc Mougeot, Benjamin R Brooks, Jennifer W Weller |
Abstract |
Sporadic Amyotrophic Lateral Sclerosis (sALS) is a devastating, complex disease of unknown etiology. We studied this disease with microarray technology to capture as much biological complexity as possible. The Affymetrix-focused BaFL pipeline takes into account problems with probes that arise from physical and biological properties, so we adapted it to handle the long-oligonucleotide probes on our arrays (hence LO-BaFL). The revised method was tested against a validated array experiment and then used in a meta-analysis of peripheral white blood cells from healthy control samples in two experiments. We predicted differentially expressed (DE) genes in our sALS data, combining the results obtained using the TM4 suite of tools with those from the LO-BaFL method. Those predictions were tested using qRT-PCR assays. |
X Demographics
Geographical breakdown
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France | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 50% |
Scientists | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 22 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 7 | 32% |
Researcher | 6 | 27% |
Student > Ph. D. Student | 3 | 14% |
Student > Postgraduate | 2 | 9% |
Student > Master | 1 | 5% |
Other | 2 | 9% |
Unknown | 1 | 5% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 7 | 32% |
Biochemistry, Genetics and Molecular Biology | 6 | 27% |
Computer Science | 2 | 9% |
Engineering | 2 | 9% |
Social Sciences | 1 | 5% |
Other | 2 | 9% |
Unknown | 2 | 9% |