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E2 multimeric scaffold for vaccine formulation: immune response by intranasal delivery and transcriptome profile of E2-pulsed dendritic cells

Overview of attention for article published in BMC Microbiology, July 2016
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3 tweeters

Citations

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6 Dimensions

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16 Mendeley
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Title
E2 multimeric scaffold for vaccine formulation: immune response by intranasal delivery and transcriptome profile of E2-pulsed dendritic cells
Published in
BMC Microbiology, July 2016
DOI 10.1186/s12866-016-0772-x
Pubmed ID
Authors

Maria Trovato, Francesco Maurano, Luciana D’Apice, Valerio Costa, Rossella Sartorius, Fausta Cuccaro, Sean P. McBurney, Shelly J. Krebs, Antonella Prisco, Alfredo Ciccodicola, Mauro Rossi, Nancy L. Haigwood, Piergiuseppe De Berardinis

Abstract

The E2 multimeric scaffold represents a powerful delivery system able to elicit robust humoral and cellular immune responses upon systemic administrations. Here recombinant E2 scaffold displaying the third variable loop of HIV-1 Envelope gp120 glycoprotein was administered via mucosa, and the mucosal and systemic immune responses were analysed. To gain further insights into the molecular mechanisms that orchestrate the immune response upon E2 vaccination, we analysed the transcriptome profile of dendritic cells (DCs) exposed to the E2 scaffold with the aim to define a specific gene expression signature for E2-primed immune responses. The in vivo immunogenicity and the potential of E2 scaffold as a mucosal vaccine candidate were investigated in BALB/c mice vaccinated via the intranasal route. Fecal and systemic antigen-specific IgA antibodies, cytokine-producing CD4(+) and CD8(+) cells were induced assessing the immunogenicity of E2 particles via intranasal administration. The cytokine analysis identified a mixed T-helper cell response, while the systemic antibody response showed a prevalence of IgG1 isotype indicative of a polarized Th2-type immune response. RNA-Sequencing analysis revealed that E2 scaffold up-regulates in DCs transcriptional regulators of the Th2-polarizing cell response, defining a type 2 DC transcriptomic signature. The current study provides experimental evidence to the possible application of E2 scaffold as antigen delivery system for mucosal immunization and taking advantages of genome-wide approach dissects the type of response induced by E2 particles.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 16 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 19%
Professor 2 13%
Student > Doctoral Student 2 13%
Student > Master 2 13%
Other 2 13%
Other 1 6%
Unknown 4 25%
Readers by discipline Count As %
Agricultural and Biological Sciences 5 31%
Biochemistry, Genetics and Molecular Biology 2 13%
Medicine and Dentistry 1 6%
Materials Science 1 6%
Engineering 1 6%
Other 0 0%
Unknown 6 38%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 June 2017.
All research outputs
#6,492,948
of 11,342,318 outputs
Outputs from BMC Microbiology
#749
of 1,601 outputs
Outputs of similar age
#113,209
of 242,282 outputs
Outputs of similar age from BMC Microbiology
#45
of 92 outputs
Altmetric has tracked 11,342,318 research outputs across all sources so far. This one is in the 40th percentile – i.e., 40% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,601 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 49th percentile – i.e., 49% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 242,282 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 50% of its contemporaries.
We're also able to compare this research output to 92 others from the same source and published within six weeks on either side of this one. This one is in the 47th percentile – i.e., 47% of its contemporaries scored the same or lower than it.