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Indication of nerve growth factor binding components from herbal extracts by HerboChip: a platform for drug screening on a chip

Overview of attention for article published in Chinese Medicine, July 2016
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Title
Indication of nerve growth factor binding components from herbal extracts by HerboChip: a platform for drug screening on a chip
Published in
Chinese Medicine, July 2016
DOI 10.1186/s13020-016-0107-8
Pubmed ID
Authors

Pinky Sum Chi Lee, Laura Minglu Zhang, Artemis Lu Yan, Kelly Yin Ching Lam, Tina Tingxia Dong, Huangquan Lin, Gallant Kar Lun Chan, Karl Wah Keung Tsim

Abstract

HerboChip is an array of different fractions deriving from herbal extracts. This study aimed to identify effective components from Chinese medicine (CM) that interact with nerve growth factor (NGF) as a target using HerboChip. Fifty types of CM that are traditionally used as remedies for emotion imbalance were selected and extracted with 50 % ethanol. Biotinylated-NGF was hybridized with over 300 chips coated with different HPLC-separated fractions from CM extracts and straptavidin-Cy5 was used to identify the NGF-bound fractions. Over 300 chips were screened within a week, and 17 positive hits were identified. The interaction of the identified herbal extracts with NGF was confirmed in cultured PC12 cells. Co-application of NGF and herbal extract interfered with NGF-induced expression of neurofilaments, including NF68 and NF200 in cell cultures. Western blot analysis comparing the intensity of phosphorylated cAMP response element-binding protein (CREB) over total CREB showed NGF-induced CREB phosphorylation was modulated by the identified herbal extracts. Five CM herbs showed activating activities on the NGF response and nine CM herbs showed inhibiting activities. The current result supported the applicability of HerboChip for screening NGF binding components from herbal extracts.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 6 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 50%
Professor 1 17%
Student > Master 1 17%
Unknown 1 17%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 17%
Nursing and Health Professions 1 17%
Agricultural and Biological Sciences 1 17%
Medicine and Dentistry 1 17%
Unknown 2 33%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 July 2016.
All research outputs
#7,508,428
of 9,727,557 outputs
Outputs from Chinese Medicine
#174
of 257 outputs
Outputs of similar age
#186,575
of 264,716 outputs
Outputs of similar age from Chinese Medicine
#5
of 9 outputs
Altmetric has tracked 9,727,557 research outputs across all sources so far. This one is in the 12th percentile – i.e., 12% of other outputs scored the same or lower than it.
So far Altmetric has tracked 257 research outputs from this source. They receive a mean Attention Score of 4.7. This one is in the 17th percentile – i.e., 17% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 264,716 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 16th percentile – i.e., 16% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 9 others from the same source and published within six weeks on either side of this one. This one has scored higher than 4 of them.