Title |
Role of endothelial microRNA 155 on capillary leakage in systemic inflammation
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Published in |
Critical Care, February 2021
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DOI | 10.1186/s13054-021-03500-0 |
Pubmed ID | |
Authors |
Valerie Etzrodt, Temitayo O. Idowu, Heiko Schenk, Benjamin Seeliger, Antje Prasse, Kristina Thamm, Thorben Pape, Janina Müller-Deile, Matijs van Meurs, Thomas Thum, Ankita Garg, Robert Geffers, Klaus Stahl, Samir M. Parikh, Hermann Haller, Sascha David |
Abstract |
Capillary leakage is a key contributor to the pathological host response to infections. The underlying mechanisms remain incompletely understood, and the role of microRNAs (MIR) has not been investigated in detail. We hypothesized that specific MIRs might be regulated directly in the endothelium thereby contributing to vascular leakage. SmallRNA sequencing of endotoxemic murine pulmonary endothelial cells (ECs) was done to detect regulated vascular MIRs. In vivo models: transgenic zebrafish (flk1:mCherry/l-fabp:eGFP-DPB), knockout/wildtype mouse (B6.Cg-Mir155tm1.1Rsky/J); disease models: LPS 17.5 mg/kgBW and cecal ligation and puncture (CLP); in vitro models: stimulated human umbilical vein EC (HUVECs), transendothelial electrical resistance. Endothelial MIR155 was identified as a promising candidate in endotoxemic murine pulmonary ECs (25 × upregulation). Experimental overexpression in a transgenic zebrafish line and in HUVECs was sufficient to induce spontaneous vascular leakage. To the contrary, genetic MIR155 reduction protects against permeability both in vitro and in endotoxemia in vivo in MIR155 heterozygote knockout mice thereby improving survival by 40%. A tight junction protein, Claudin-1, was down-regulated both in endotoxemia and by experimental MIR155 overexpression. Translationally, MIR155 was detectable at high levels in bronchoalveolar fluid of patients with ARDS compared to healthy human subjects. We found that MIR155 is upregulated in the endothelium in mouse and men as part of a systemic inflammatory response and might contribute to the pathophysiology of vascular leakage in a Claudin-1-dependent manner. Future studies have to clarify whether MIR155 could be a potential therapeutic target. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Germany | 2 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 50% |
Practitioners (doctors, other healthcare professionals) | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 23 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Doctoral Student | 3 | 13% |
Researcher | 3 | 13% |
Student > Ph. D. Student | 3 | 13% |
Librarian | 2 | 9% |
Student > Bachelor | 1 | 4% |
Other | 2 | 9% |
Unknown | 9 | 39% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 9 | 39% |
Agricultural and Biological Sciences | 2 | 9% |
Biochemistry, Genetics and Molecular Biology | 1 | 4% |
Immunology and Microbiology | 1 | 4% |
Engineering | 1 | 4% |
Other | 0 | 0% |
Unknown | 9 | 39% |