Title |
Genotypic characterization of drug resistant Mycobacterium tuberculosis in Quebec, 2002-2012
|
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Published in |
BMC Microbiology, July 2016
|
DOI | 10.1186/s12866-016-0786-4 |
Pubmed ID | |
Authors |
Joanna Spinato, Élyse Boivin, Émilie Bélanger-Trudelle, Huguette Fauchon, Cécile Tremblay, Hafid Soualhine |
Abstract |
The increasing emergence of drug-resistant tuberculosis presents a threat to the effective control of tuberculosis (TB). Rapid detection of drug-resistance is more important than ever to address this scourge. The purpose of this study was to genotypically characterize the first-line antitubercular drug-resistant isolates collected over 11 years in Quebec. The main mutations found in our resistant strains collection (n = 225) include: the S315T substitution in katG (50.2 %), the -15 C/T mutation in the inhA promoter (29 %); the S531L substitution in rpoB (43 %); the deletion 8 bp 446 / + R140S in pncA (72.9 %); the M306I (35.7 %) and M306V (21.4 %) substitutions in embB. Ten of the mutations in katG and 4 mutations identified in pncA were previously undescribed. Screening of mutations conferring resistance to first-line antituberculous drugs using DNA-sequencing approach seems to be feasible and would drastically shorten the time to determine the resistance profile compared to the proportion method. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 33% |
Venezuela, Bolivarian Republic of | 1 | 33% |
Unknown | 1 | 33% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 33% |
Practitioners (doctors, other healthcare professionals) | 1 | 33% |
Scientists | 1 | 33% |
Mendeley readers
Geographical breakdown
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Unknown | 30 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 6 | 20% |
Student > Postgraduate | 4 | 13% |
Researcher | 4 | 13% |
Student > Master | 3 | 10% |
Other | 3 | 10% |
Other | 5 | 17% |
Unknown | 5 | 17% |
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Immunology and Microbiology | 6 | 20% |
Biochemistry, Genetics and Molecular Biology | 4 | 13% |
Agricultural and Biological Sciences | 2 | 7% |
Nursing and Health Professions | 1 | 3% |
Other | 2 | 7% |
Unknown | 8 | 27% |