Title |
i-Tracker: For quantitative proteomics using iTRAQ™
|
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Published in |
BMC Genomics, October 2005
|
DOI | 10.1186/1471-2164-6-145 |
Pubmed ID | |
Authors |
Ian P Shadforth, Tom PJ Dunkley, Kathryn S Lilley, Conrad Bessant |
Abstract |
iTRAQ technology for protein quantitation using mass spectrometry is a recent, powerful means of determining relative protein levels in up to four samples simultaneously. Although protein identification of samples generated using iTRAQ may be carried out using any current identification software, the quantitation calculations have been restricted to the ProQuant software supplied by Applied Biosciences. i-Tracker software has been developed to extract reporter ion peak ratios from non-centroided tandem MS peak lists in a format easily linked to the results of protein identification tools such as Mascot and Sequest. Such functionality is currently not provided by ProQuant, which is restricted to matching quantitative information to the peptide identifications from Applied Biosciences' Interrogator software. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 5 | 2% |
United Kingdom | 4 | 2% |
South Africa | 4 | 2% |
Germany | 3 | 1% |
France | 2 | <1% |
Netherlands | 1 | <1% |
Italy | 1 | <1% |
Mexico | 1 | <1% |
Sweden | 1 | <1% |
Other | 0 | 0% |
Unknown | 192 | 90% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 60 | 28% |
Researcher | 59 | 28% |
Student > Master | 34 | 16% |
Professor > Associate Professor | 15 | 7% |
Student > Doctoral Student | 9 | 4% |
Other | 24 | 11% |
Unknown | 13 | 6% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 115 | 54% |
Biochemistry, Genetics and Molecular Biology | 31 | 14% |
Chemistry | 24 | 11% |
Computer Science | 8 | 4% |
Pharmacology, Toxicology and Pharmaceutical Science | 4 | 2% |
Other | 20 | 9% |
Unknown | 12 | 6% |