ENU-induced phenovariance in mice: inferences from 587 mutations

Carrie N Arnold, Michael J Barnes, Michael Berger, Amanda L Blasius, Katharina Brandl, Ben Croker, Karine Crozat, Xin Du, Celine Eidenschenk, Philippe Georgel, Kasper Hoebe, Hua Huang, Zhengfan Jiang, Philippe Krebs, Diantha La Vine, Xiaohong Li, Stephen Lyon, Eva Marie Y Moresco, Anne R Murray, Daniel L Popkin, Sophie Rutschmann, Owen M Siggs, Nora G Smart, Lei Sun, Koichi Tabeta, Victoria Webster, Wataru Tomisato, Sungyong Won, Yu Xia, Nengming Xiao, Bruce Beutler

We present a compendium of N-ethyl-N-nitrosourea (ENU)-induced mouse mutations, identified in our laboratory over a period of 10 years either on the basis of phenotype or whole genome and/or whole exome sequencing, and archived in the Mutagenetix database. Our purpose is threefold: 1) to formally describe many point mutations, including those that were not previously disclosed in peer-reviewed publications; 2) to assess the characteristics of these mutations; and 3) to estimate the likelihood that a missense mutation induced by ENU will create a detectable phenotype.