Title |
Larval midgut modifications associated with Bti resistance in the yellow fever mosquito using proteomic and transcriptomic approaches
|
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Published in |
BMC Genomics, June 2012
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DOI | 10.1186/1471-2164-13-248 |
Pubmed ID | |
Authors |
Guillaume Tetreau, Krishnareddy Bayyareddy, Christopher M Jones, Renaud Stalinski, Muhammad A Riaz, Margot Paris, Jean-Philippe David, Michael J Adang, Laurence Després |
Abstract |
Bacillus thuringiensis var. israelensis (Bti) is a natural larval mosquito pathogen producing pore-forming toxins targeting the midgut of Diptera larvae. It is used worldwide for mosquito control. Resistance mechanisms of an Aedes aegypti laboratory strain selected for 30 generations with field-collected leaf litter containing Bti toxins were investigated in larval midguts at two levels: 1. gene transcription using DNA microarray and RT-qPCR and 2. differential expression of brush border membrane proteins using DIGE (Differential In Gel Electrophoresis). |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Colombia | 1 | <1% |
France | 1 | <1% |
United Kingdom | 1 | <1% |
Tunisia | 1 | <1% |
Slovenia | 1 | <1% |
Unknown | 112 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 25 | 21% |
Student > Master | 18 | 15% |
Researcher | 16 | 14% |
Student > Doctoral Student | 11 | 9% |
Student > Bachelor | 8 | 7% |
Other | 25 | 21% |
Unknown | 14 | 12% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 60 | 51% |
Biochemistry, Genetics and Molecular Biology | 15 | 13% |
Environmental Science | 6 | 5% |
Immunology and Microbiology | 5 | 4% |
Unspecified | 5 | 4% |
Other | 11 | 9% |
Unknown | 15 | 13% |