Title |
Bacillus coagulans GBI-30, 6086 limits the recurrence of Clostridium difficile-Induced colitis following vancomycin withdrawal in mice
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Published in |
Gut Pathogens, October 2012
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DOI | 10.1186/1757-4749-4-13 |
Pubmed ID | |
Authors |
Leo R Fitzpatrick, Jeffrey S Small, Wallace H Greene, Kelly D Karpa, Sean Farmer, David Keller |
Abstract |
Recently, we found that the probiotic strain Bacillus coagulans GBI-30, 6086 (GanedenBC30) improved indices of Clostridium difficile (C. difficile)-induced colitis in mice (Fitzpatrick et al., Gut Pathogens, 2011). Our goal was to determine if BC30 could also prevent the recurrence of C. difficile-induced colitis in mice, following initial treatment with vancomycin. During study days 0 through 5, mice were treated with antibiotics. On day 6, the C. difficile strain VPI 10463 was given by oro-gastric gavage at ≈ 5x104 CFU to induce colitis. Mice were treated on study days 6 to 10 with vancomycin (50 mg/kg) (vanco) or vehicle (saline) by gavage. On days 10 to16, mice were dosed by gavage with saline vehicle or BC30 (2 x 109 CFU per day). Mice were monitored for mortality, weight loss and diarrhea. On study days 14, 16 and 17, stools and colons were collected for analyzing other parameters of colitis. |
X Demographics
Geographical breakdown
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United States | 3 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 49 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Other | 7 | 14% |
Student > Ph. D. Student | 6 | 12% |
Researcher | 5 | 10% |
Student > Bachelor | 3 | 6% |
Student > Master | 3 | 6% |
Other | 7 | 14% |
Unknown | 18 | 37% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 8 | 16% |
Biochemistry, Genetics and Molecular Biology | 7 | 14% |
Medicine and Dentistry | 7 | 14% |
Immunology and Microbiology | 5 | 10% |
Psychology | 1 | 2% |
Other | 3 | 6% |
Unknown | 18 | 37% |