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Improved hybrid de novo genome assembly of domesticated apple (Malus x domestica)

Overview of attention for article published in Giga Science, August 2016
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (93rd percentile)
  • Above-average Attention Score compared to outputs of the same age and source (54th percentile)

Mentioned by

news
1 news outlet
blogs
1 blog
twitter
32 X users
peer_reviews
1 peer review site
facebook
2 Facebook pages

Citations

dimensions_citation
54 Dimensions

Readers on

mendeley
82 Mendeley
citeulike
1 CiteULike
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Title
Improved hybrid de novo genome assembly of domesticated apple (Malus x domestica)
Published in
Giga Science, August 2016
DOI 10.1186/s13742-016-0139-0
Pubmed ID
Authors

Xuewei Li, Ling Kui, Jing Zhang, Yinpeng Xie, Liping Wang, Yan Yan, Na Wang, Jidi Xu, Cuiying Li, Wen Wang, Steve van Nocker, Yang Dong, Fengwang Ma, Qingmei Guan

Abstract

Domesticated apple (Malus × domestica Borkh) is a popular temperate fruit with high nutrient levels and diverse flavors. In 2012, global apple production accounted for at least one tenth of all harvested fruits. A high-quality apple genome assembly is crucial for the selection and breeding of new cultivars. Currently, a single reference genome is available for apple, assembled from 16.9 × genome coverage short reads via Sanger and 454 sequencing technologies. Although a useful resource, this assembly covers only ~89 % of the non-repetitive portion of the genome, and has a relatively short (16.7 kb) contig N50 length. These downsides make it difficult to apply this reference in transcriptive or whole-genome re-sequencing analyses. Here we present an improved hybrid de novo genomic assembly of apple (Golden Delicious), which was obtained from 76 Gb (~102 × genome coverage) Illumina HiSeq data and 21.7 Gb (~29 × genome coverage) PacBio data. The final draft genome is approximately 632.4 Mb, representing ~ 90 % of the estimated genome. The contig N50 size is 111,619 bp, representing a 7 fold improvement. Further annotation analyses predicted 53,922 protein-coding genes and 2,765 non-coding RNA genes. The new apple genome assembly will serve as a valuable resource for investigating complex apple traits at the genomic level. It is not only suitable for genome editing and gene cloning, but also for RNA-seq and whole-genome re-sequencing studies.

X Demographics

X Demographics

The data shown below were collected from the profiles of 32 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 82 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 1%
Netherlands 1 1%
Poland 1 1%
Unknown 79 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 17 21%
Researcher 15 18%
Student > Master 10 12%
Student > Postgraduate 7 9%
Student > Doctoral Student 5 6%
Other 13 16%
Unknown 15 18%
Readers by discipline Count As %
Agricultural and Biological Sciences 39 48%
Biochemistry, Genetics and Molecular Biology 20 24%
Computer Science 4 5%
Nursing and Health Professions 1 1%
Unspecified 1 1%
Other 1 1%
Unknown 16 20%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 33. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 December 2016.
All research outputs
#1,226,381
of 25,578,098 outputs
Outputs from Giga Science
#197
of 1,174 outputs
Outputs of similar age
#23,176
of 379,171 outputs
Outputs of similar age from Giga Science
#6
of 11 outputs
Altmetric has tracked 25,578,098 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 95th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,174 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 21.7. This one has done well, scoring higher than 83% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 379,171 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 93% of its contemporaries.
We're also able to compare this research output to 11 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 54% of its contemporaries.