Title |
Combining proteomics and transcriptome sequencing to identify active plant-cell-wall-degrading enzymes in a leaf beetle
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Published in |
BMC Genomics, November 2012
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DOI | 10.1186/1471-2164-13-587 |
Pubmed ID | |
Authors |
Roy Kirsch, Natalie Wielsch, Heiko Vogel, Aleš Svatoš, David G Heckel, Yannick Pauchet |
Abstract |
The primary plant cell wall is a complex mixture of polysaccharides and proteins encasing living plant cells. Among these polysaccharides, cellulose is the most abundant and useful biopolymer present on earth. These polysaccharides also represent a rich source of energy for organisms which have evolved the ability to degrade them. A growing body of evidence suggests that phytophagous beetles, mainly species from the superfamilies Chrysomeloidea and Curculionoidea, possess endogenous genes encoding complex and diverse families of so-called plant cell wall degrading enzymes (PCWDEs). The presence of these genes in phytophagous beetles may have been a key element in their success as herbivores. Here, we combined a proteomics approach and transcriptome sequencing to identify PCWDEs present in larval gut contents of the mustard leaf beetle, Phaedon cochleariae. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 2 | 67% |
Spain | 1 | 33% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 67% |
Scientists | 1 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Germany | 2 | 3% |
France | 1 | 1% |
Austria | 1 | 1% |
Sweden | 1 | 1% |
Mexico | 1 | 1% |
Japan | 1 | 1% |
Unknown | 73 | 91% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 22 | 28% |
Researcher | 15 | 19% |
Student > Doctoral Student | 6 | 8% |
Student > Postgraduate | 5 | 6% |
Student > Master | 5 | 6% |
Other | 16 | 20% |
Unknown | 11 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 46 | 57% |
Biochemistry, Genetics and Molecular Biology | 12 | 15% |
Chemistry | 4 | 5% |
Environmental Science | 3 | 4% |
Unspecified | 1 | 1% |
Other | 2 | 3% |
Unknown | 12 | 15% |