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Grape compounds suppress colon cancer stem cells in vitro and in a rodent model of colon carcinogenesis

Overview of attention for article published in BMC Complementary and Alternative Medicine, August 2016
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • Among the highest-scoring outputs from this source (#42 of 2,377)
  • High Attention Score compared to outputs of the same age (95th percentile)
  • High Attention Score compared to outputs of the same age and source (96th percentile)

Mentioned by

news
6 news outlets
twitter
8 tweeters
facebook
6 Facebook pages

Citations

dimensions_citation
41 Dimensions

Readers on

mendeley
78 Mendeley
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Title
Grape compounds suppress colon cancer stem cells in vitro and in a rodent model of colon carcinogenesis
Published in
BMC Complementary and Alternative Medicine, August 2016
DOI 10.1186/s12906-016-1254-2
Pubmed ID
Authors

Lavanya Reddivari, Venkata Charepalli, Sridhar Radhakrishnan, Ramakrishna Vadde, Ryan J. Elias, Joshua D. Lambert, Jairam K. P. Vanamala

Abstract

We have previously shown that the grape bioactive compound resveratrol (RSV) potentiates grape seed extract (GSE)-induced colon cancer cell apoptosis at physiologically relevant concentrations. However, RSV-GSE combination efficacy against colon cancer stem cells (CSCs), which play a key role in chemotherapy and radiation resistance, is not known. We tested the anti-cancer efficacy of the RSV-GSE against colon CSCs using isolated human colon CSCs in vitro and an azoxymethane-induced mouse model of colon carcinogenesis in vivo. RSV-GSE suppressed tumor incidence similar to sulindac, without any gastrointestinal toxicity. Additionally, RSV-GSE treatment reduced the number of crypts containing cells with nuclear β-catenin (an indicator of colon CSCs) via induction of apoptosis. In vitro, RSV-GSE suppressed - proliferation, sphere formation, nuclear translocation of β-catenin (a critical regulator of CSC proliferation) similar to sulindac in isolated human colon CSCs. RSV-GSE, but not sulindac, suppressed downstream protein levels of Wnt/β-catenin pathway, c-Myc and cyclin D1. RSV-GSE also induced mitochondrial-mediated apoptosis in colon CSCs characterized by elevated p53, Bax/Bcl-2 ratio and cleaved PARP. Furthermore, shRNA-mediated knockdown of p53, a tumor suppressor gene, in colon CSCs did not alter efficacy of RSV-GSE. The suppression of Wnt/β-catenin signaling and elevated mitochondrial-mediated apoptosis in colon CSCs support potential clinical testing/application of grape bioactives for colon cancer prevention and/or therapy.

Twitter Demographics

The data shown below were collected from the profiles of 8 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 78 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 78 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 15 19%
Student > Ph. D. Student 10 13%
Researcher 9 12%
Student > Bachelor 8 10%
Other 6 8%
Other 15 19%
Unknown 15 19%
Readers by discipline Count As %
Agricultural and Biological Sciences 21 27%
Biochemistry, Genetics and Molecular Biology 12 15%
Medicine and Dentistry 12 15%
Chemistry 4 5%
Immunology and Microbiology 3 4%
Other 6 8%
Unknown 20 26%

Attention Score in Context

This research output has an Altmetric Attention Score of 52. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 July 2017.
All research outputs
#243,283
of 11,424,220 outputs
Outputs from BMC Complementary and Alternative Medicine
#42
of 2,377 outputs
Outputs of similar age
#11,537
of 263,106 outputs
Outputs of similar age from BMC Complementary and Alternative Medicine
#4
of 102 outputs
Altmetric has tracked 11,424,220 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 97th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,377 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.8. This one has done particularly well, scoring higher than 98% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 263,106 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 95% of its contemporaries.
We're also able to compare this research output to 102 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 96% of its contemporaries.