Title |
Evidence for Vpr-dependent HIV-1 Replication in Human CD4+CEM.NKR T-Cells
|
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Published in |
Retrovirology, November 2012
|
DOI | 10.1186/1742-4690-9-93 |
Pubmed ID | |
Authors |
Tao Zhou, Ying Dang, Jacob J Baker, Jiajun Zhou, Yong-Hui Zheng |
Abstract |
Vpr is exclusively expressed in primate lentiviruses and contributes to viral replication and disease progression in vivo. HIV-1 Vpr has two major activities in vitro: arrest of cell cycle in the G2 phase (G2 arrest), and enhancement of viral replication in macrophages. Previously, we reported a potent HIV-1 restriction in the human CD4+ CEM.NKR (NKR) T cells, where wild-type (WT) HIV-1 replication was inhibited by almost 1,000-fold. From the parental NKR cells, we isolated eight clones by limiting dilution. These clones showed three levels of resistance to the WT HIV-1 infection: non-permissive (NP), semi-permissive (SP), and permissive (P). Here, we compared the replication of WT, Vif-defective, Vpr-defective, and Vpu-defective viruses in these cells. |
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United States | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
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South Africa | 1 | 3% |
Unknown | 31 | 97% |
Demographic breakdown
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Researcher | 10 | 31% |
Student > Ph. D. Student | 9 | 28% |
Student > Bachelor | 3 | 9% |
Student > Postgraduate | 3 | 9% |
Student > Master | 1 | 3% |
Other | 3 | 9% |
Unknown | 3 | 9% |
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Nursing and Health Professions | 1 | 3% |
Other | 0 | 0% |
Unknown | 4 | 13% |