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Optimisation of sample storage and DNA extraction for human gut microbiota studies​

Overview of attention for article published in BMC Microbiology, May 2021
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (55th percentile)

Mentioned by

twitter
6 tweeters

Citations

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3 Dimensions

Readers on

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17 Mendeley
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Title
Optimisation of sample storage and DNA extraction for human gut microbiota studies​
Published in
BMC Microbiology, May 2021
DOI 10.1186/s12866-021-02233-y
Pubmed ID
Authors

Jekaterina Kazantseva, Esther Malv, Aleksei Kaleda, Aili Kallastu, Anne Meikas

Abstract

New developments in next-generation sequencing technologies and massive data received from this approach open wide prospects for personalised medicine and nutrition studies. Metagenomic analysis of the gut microbiota is paramount for the characterization of human health and wellbeing. Despite the intensive research, there is a huge gap and inconsistency between different studies due to the non-standardised and biased pipeline. Methodical and systemic understanding of every stage in the process is necessary to overcome all bottlenecks and grey zones of gut microbiota studies, where all details and interactions between processes are important. Here we show that an inexpensive, but reliable iSeq 100 platform is an excellent tool to perform the analysis of the human gut microbiota by amplicon sequencing of the 16 S rRNA gene. Two commercial DNA extraction kits and different starting materials performed similarly regarding the taxonomic distribution of identified bacteria. DNA/RNA Shield reagent proved to be a reliable solution for stool samples collection, preservation, and storage, as the storage of faecal material in DNA/RNA Shield for three weeks at different temperatures and thawing cycles had a low impact on the bacterial distribution. Altogether, a thoroughly elaborated pipeline with close attention to details ensures high reproducibility with significant biological but not technical variations.

Twitter Demographics

The data shown below were collected from the profiles of 6 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 17 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 24%
Student > Ph. D. Student 2 12%
Student > Doctoral Student 1 6%
Other 1 6%
Unspecified 1 6%
Other 1 6%
Unknown 7 41%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 18%
Agricultural and Biological Sciences 2 12%
Mathematics 1 6%
Environmental Science 1 6%
Computer Science 1 6%
Other 2 12%
Unknown 7 41%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 May 2021.
All research outputs
#11,067,045
of 19,508,584 outputs
Outputs from BMC Microbiology
#1,072
of 2,880 outputs
Outputs of similar age
#150,669
of 343,201 outputs
Outputs of similar age from BMC Microbiology
#1
of 1 outputs
Altmetric has tracked 19,508,584 research outputs across all sources so far. This one is in the 42nd percentile – i.e., 42% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,880 research outputs from this source. They receive a mean Attention Score of 3.9. This one has gotten more attention than average, scoring higher than 61% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 343,201 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 55% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them