Title |
Improved base-calling and quality scores for 454 sequencing based on a Hurdle Poisson model
|
---|---|
Published in |
BMC Bioinformatics, November 2012
|
DOI | 10.1186/1471-2105-13-303 |
Pubmed ID | |
Authors |
Kristof De Beuf, Joachim De Schrijver, Olivier Thas, Wim Van Criekinge, Rafael A Irizarry, Lieven Clement |
Abstract |
454 pyrosequencing is a commonly used massively parallel DNA sequencing technology with a wide variety of application fields such as epigenetics, metagenomics and transcriptomics. A well-known problem of this platform is its sensitivity to base-calling insertion and deletion errors, particularly in the presence of long homopolymers. In addition, the base-call quality scores are not informative with respect to whether an insertion or a deletion error is more likely. Surprisingly, not much effort has been devoted to the development of improved base-calling methods and more intuitive quality scores for this platform. |
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Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 20% |
Germany | 1 | 20% |
Norway | 1 | 20% |
Sweden | 1 | 20% |
Unknown | 1 | 20% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 4 | 80% |
Members of the public | 1 | 20% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Brazil | 4 | 7% |
United States | 2 | 3% |
Germany | 1 | 2% |
Sweden | 1 | 2% |
Kenya | 1 | 2% |
Spain | 1 | 2% |
Argentina | 1 | 2% |
Unknown | 50 | 82% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 20 | 33% |
Student > Ph. D. Student | 15 | 25% |
Student > Master | 5 | 8% |
Student > Bachelor | 3 | 5% |
Professor | 3 | 5% |
Other | 11 | 18% |
Unknown | 4 | 7% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 37 | 61% |
Mathematics | 4 | 7% |
Biochemistry, Genetics and Molecular Biology | 4 | 7% |
Computer Science | 4 | 7% |
Immunology and Microbiology | 3 | 5% |
Other | 3 | 5% |
Unknown | 6 | 10% |