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The Golgi apparatus acts as a platform for TBK1 activation after viral RNA sensing

Overview of attention for article published in BMC Biology, August 2016
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5 tweeters

Citations

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42 Dimensions

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61 Mendeley
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Title
The Golgi apparatus acts as a platform for TBK1 activation after viral RNA sensing
Published in
BMC Biology, August 2016
DOI 10.1186/s12915-016-0292-z
Pubmed ID
Authors

Marie Pourcelot, Naima Zemirli, Leandro Silva Da Costa, Roxane Loyant, Dominique Garcin, Damien Vitour, Ivana Munitic, Aimé Vazquez, Damien Arnoult

Abstract

After viral infection and the stimulation of some pattern-recognition receptors, TANK-binding kinase I (TBK1) is activated by K63-linked polyubiquitination followed by trans-autophosphorylation. While the activated TBK1 induces type I interferon production by phosphorylating the transcription factor IRF3, the precise molecular mechanisms underlying TBK1 activation remain unclear. We report here the localization of the ubiquitinated and phosphorylated active form of TBK1 to the Golgi apparatus after the stimulation of RIG-I-like receptors (RLRs) or Toll-like receptor-3 (TLR3), due to TBK1 K63-linked ubiquitination on lysine residues 30 and 401. The ubiquitin-binding protein optineurin (OPTN) recruits ubiquitinated TBK1 to the Golgi apparatus, leading to the formation of complexes in which TBK1 is activated by trans-autophosphorylation. Indeed, OPTN deficiency in various cell lines and primary cells impairs TBK1 targeting to the Golgi apparatus and its activation following RLR or TLR3 stimulation. Interestingly, the Bluetongue virus NS3 protein binds OPTN at the Golgi apparatus, neutralizing its activity and thereby decreasing TBK1 activation and downstream signaling. Our results highlight an unexpected role of the Golgi apparatus in innate immunity as a key subcellular gateway for TBK1 activation after RNA virus infection.

Twitter Demographics

The data shown below were collected from the profiles of 5 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 61 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
France 1 2%
Unknown 60 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 18 30%
Student > Master 10 16%
Researcher 9 15%
Student > Doctoral Student 5 8%
Student > Bachelor 3 5%
Other 5 8%
Unknown 11 18%
Readers by discipline Count As %
Agricultural and Biological Sciences 17 28%
Biochemistry, Genetics and Molecular Biology 13 21%
Immunology and Microbiology 10 16%
Medicine and Dentistry 3 5%
Social Sciences 1 2%
Other 2 3%
Unknown 15 25%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 July 2021.
All research outputs
#11,323,308
of 19,233,288 outputs
Outputs from BMC Biology
#1,345
of 1,664 outputs
Outputs of similar age
#132,241
of 275,021 outputs
Outputs of similar age from BMC Biology
#1
of 1 outputs
Altmetric has tracked 19,233,288 research outputs across all sources so far. This one is in the 40th percentile – i.e., 40% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,664 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 20.1. This one is in the 18th percentile – i.e., 18% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 275,021 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 50% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them