Title |
Identification of proteins binding coding and non-coding human RNAs using protein microarrays
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Published in |
BMC Genomics, November 2012
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DOI | 10.1186/1471-2164-13-633 |
Pubmed ID | |
Authors |
Zurab Siprashvili, Dan E Webster, Markus Kretz, Danielle Johnston, John L Rinn, Howard Y Chang, Paul A Khavari |
Abstract |
The regulation and function of mammalian RNAs has been increasingly appreciated to operate via RNA-protein interactions. With the recent discovery of thousands of novel human RNA molecules by high-throughput RNA sequencing, efficient methods to uncover RNA-protein interactions are urgently required. Existing methods to study proteins associated with a given RNA are laborious and require substantial amounts of cell-derived starting material. To overcome these limitations, we have developed a rapid and large-scale approach to characterize binding of in vitro transcribed labeled RNA to ~9,400 human recombinant proteins spotted on protein microarrays. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 2 | 67% |
Unknown | 1 | 33% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 67% |
Scientists | 1 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United Kingdom | 2 | 2% |
Germany | 1 | 1% |
Malaysia | 1 | 1% |
South Africa | 1 | 1% |
France | 1 | 1% |
Japan | 1 | 1% |
United States | 1 | 1% |
Unknown | 89 | 92% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 30 | 31% |
Researcher | 23 | 24% |
Student > Master | 11 | 11% |
Student > Bachelor | 6 | 6% |
Student > Postgraduate | 5 | 5% |
Other | 15 | 15% |
Unknown | 7 | 7% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 47 | 48% |
Biochemistry, Genetics and Molecular Biology | 24 | 25% |
Engineering | 6 | 6% |
Medicine and Dentistry | 4 | 4% |
Computer Science | 3 | 3% |
Other | 4 | 4% |
Unknown | 9 | 9% |