Title |
Development of a multiplex methylation-specific PCR as candidate triage test for women with an HPV-positive cervical scrape
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Published in |
BMC Cancer, November 2012
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DOI | 10.1186/1471-2407-12-551 |
Pubmed ID | |
Authors |
Suzanne Snellenberg, Lise MA De Strooper, Albertus T Hesselink, Chris JLM Meijer, Peter JF Snijders, Daniëlle AM Heideman, Renske DM Steenbergen |
Abstract |
Quantitative methylation-specific PCR (qMSP) analysis for determining the methylation status of (candidate) tumor suppressor genes has potential as objective and valuable test to triage high-risk human papillomavirus (hrHPV) positive women in cervical screening. Particularly combined methylation analysis of a panel of genes shows most promising clinical performance, with sensitivity levels that equal or exceed that of cytology. However, the wide application of such methylation marker panels is hampered by the lack of effective multiplex assays allowing simultaneous methylation detection of various targets in a single reaction. Here, we designed and analyzed a multiplex qMSP assay for three genes whose methylation was previously found to be informative for cervical (pre)cancer (i.e. CADM1, MAL and hsa-miR-124-2) as well as a reference gene β-actin. Based on our experience, we discuss the optimization of the parameters that provide a practical approach towards multiplex qMSP design. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Netherlands | 2 | 2% |
Unknown | 92 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 21 | 22% |
Student > Master | 13 | 14% |
Student > Bachelor | 13 | 14% |
Researcher | 12 | 13% |
Other | 7 | 7% |
Other | 15 | 16% |
Unknown | 13 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 36 | 38% |
Agricultural and Biological Sciences | 17 | 18% |
Biochemistry, Genetics and Molecular Biology | 16 | 17% |
Nursing and Health Professions | 3 | 3% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 2% |
Other | 6 | 6% |
Unknown | 14 | 15% |