↓ Skip to main content

Development of a multiplex methylation-specific PCR as candidate triage test for women with an HPV-positive cervical scrape

Overview of attention for article published in BMC Cancer, November 2012
Altmetric Badge

About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (82nd percentile)
  • High Attention Score compared to outputs of the same age and source (89th percentile)

Mentioned by

twitter
2 tweeters
patent
6 patents

Citations

dimensions_citation
42 Dimensions

Readers on

mendeley
79 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Development of a multiplex methylation-specific PCR as candidate triage test for women with an HPV-positive cervical scrape
Published in
BMC Cancer, November 2012
DOI 10.1186/1471-2407-12-551
Pubmed ID
Authors

Suzanne Snellenberg, Lise MA De Strooper, Albertus T Hesselink, Chris JLM Meijer, Peter JF Snijders, Daniëlle AM Heideman, Renske DM Steenbergen

Abstract

Quantitative methylation-specific PCR (qMSP) analysis for determining the methylation status of (candidate) tumor suppressor genes has potential as objective and valuable test to triage high-risk human papillomavirus (hrHPV) positive women in cervical screening. Particularly combined methylation analysis of a panel of genes shows most promising clinical performance, with sensitivity levels that equal or exceed that of cytology. However, the wide application of such methylation marker panels is hampered by the lack of effective multiplex assays allowing simultaneous methylation detection of various targets in a single reaction. Here, we designed and analyzed a multiplex qMSP assay for three genes whose methylation was previously found to be informative for cervical (pre)cancer (i.e. CADM1, MAL and hsa-miR-124-2) as well as a reference gene β-actin. Based on our experience, we discuss the optimization of the parameters that provide a practical approach towards multiplex qMSP design.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 79 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Netherlands 2 3%
Unknown 77 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 21 27%
Researcher 11 14%
Student > Master 11 14%
Student > Bachelor 9 11%
Other 7 9%
Other 13 16%
Unknown 7 9%
Readers by discipline Count As %
Medicine and Dentistry 33 42%
Agricultural and Biological Sciences 16 20%
Biochemistry, Genetics and Molecular Biology 13 16%
Nursing and Health Professions 2 3%
Immunology and Microbiology 2 3%
Other 4 5%
Unknown 9 11%

Attention Score in Context

This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 August 2019.
All research outputs
#2,879,569
of 15,606,181 outputs
Outputs from BMC Cancer
#714
of 5,827 outputs
Outputs of similar age
#44,747
of 257,982 outputs
Outputs of similar age from BMC Cancer
#35
of 325 outputs
Altmetric has tracked 15,606,181 research outputs across all sources so far. Compared to these this one has done well and is in the 81st percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 5,827 research outputs from this source. They receive a mean Attention Score of 4.1. This one has done well, scoring higher than 87% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 257,982 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 82% of its contemporaries.
We're also able to compare this research output to 325 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 89% of its contemporaries.