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A method for assessing efficiency of bacterial cell disruption and DNA release

Overview of attention for article published in BMC Microbiology, August 2016
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (87th percentile)

Mentioned by

blogs
1 blog
twitter
11 tweeters
googleplus
1 Google+ user

Citations

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19 Dimensions

Readers on

mendeley
91 Mendeley
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Title
A method for assessing efficiency of bacterial cell disruption and DNA release
Published in
BMC Microbiology, August 2016
DOI 10.1186/s12866-016-0815-3
Pubmed ID
Authors

Olle M. de Bruin, H. C. Birnboim

Abstract

DNA-based testing is becoming the preferred method both for identifying microorganisms and for characterizing microbial communities. However, no single DNA extraction method exists that is suitable for all types of microorganisms because bacteria are variable in their susceptibility to lysis by available extraction procedures. To develop and test new DNA extraction procedures, it would be helpful to determine their efficiencies. While the amount of extracted DNA can readily be measured by different methods, calculation of true efficiency requires knowledge of the initial amount of DNA in the starting bacterial sample, which cannot be done with precision by any existing method. In the process of developing a new extraction procedure, we developed a method that can be used to determine the total amount of both DNA and RNA in bacteria. The amount of DNA can be calculated from the amount of purines released after mild acid and alkali treatment. The amount of RNA in the same extract can also be calculated from the amount of ribonucleoside monophosphates. The released purines and ribonucleoside monophosphates can be quantified by absorbance using HPLC, with reference to appropriate standards. The acid/HPLC method was used to measure the efficiency of commonly used bead-beating and chemical protocols for releasing DNA from a particularly hardy organism, Mycobacterium smegmatis as well as several other species (Bacillus subtilis vegetative cells and spores; Francisella philomiragia; Pseudomonas aeruginosa; Moraxella catarrhalis; Bacillus thuringiensis; Staphylococcus aureus). Surprisingly large differences in efficiency between methods were found. The acid/HPLC method is a new tool to determine DNA extraction efficiencies and should aid in the development of improved protocols for releasing DNA from a broad range of microorganisms.

Twitter Demographics

The data shown below were collected from the profiles of 11 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 91 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
New Zealand 1 1%
Sudan 1 1%
Unknown 89 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 25 27%
Student > Bachelor 18 20%
Student > Master 12 13%
Researcher 11 12%
Student > Doctoral Student 3 3%
Other 8 9%
Unknown 14 15%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 22 24%
Agricultural and Biological Sciences 16 18%
Immunology and Microbiology 11 12%
Environmental Science 5 5%
Chemistry 4 4%
Other 14 15%
Unknown 19 21%

Attention Score in Context

This research output has an Altmetric Attention Score of 14. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 February 2022.
All research outputs
#2,051,183
of 21,775,893 outputs
Outputs from BMC Microbiology
#146
of 3,077 outputs
Outputs of similar age
#35,667
of 286,805 outputs
Outputs of similar age from BMC Microbiology
#1
of 1 outputs
Altmetric has tracked 21,775,893 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 90th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,077 research outputs from this source. They receive a mean Attention Score of 4.0. This one has done particularly well, scoring higher than 95% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 286,805 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 87% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them