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Robust succinic acid production from crude glycerol using engineered Yarrowia lipolytica

Overview of attention for article published in Biotechnology for Biofuels, August 2016
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (64th percentile)
  • High Attention Score compared to outputs of the same age and source (83rd percentile)

Mentioned by

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1 tweeter
patent
1 patent

Citations

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91 Dimensions

Readers on

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100 Mendeley
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Title
Robust succinic acid production from crude glycerol using engineered Yarrowia lipolytica
Published in
Biotechnology for Biofuels, August 2016
DOI 10.1186/s13068-016-0597-8
Pubmed ID
Authors

Cuijuan Gao, Xiaofeng Yang, Huaimin Wang, Cristina Perez Rivero, Chong Li, Zhiyong Cui, Qingsheng Qi, Carol Sze Ki Lin

Abstract

Integrating waste management with fuels and chemical production is considered to address the food waste problem and oil crisis. Approximately, 600 million tonnes crude glycerol is produced from the biodiesel industry annually, which is a top renewable feedstock for succinic acid production. To meet the increasing demand for succinic acid production, the development of more efficient and cost-effective production methods is urgently needed. Herein, we have proposed a new strategy for integration of both biodiesel and SA production in a biorefinery unit by construction of an aerobic yeast Yarrowia lipolytica with a deletion in the gene coding succinate dehydrogenase subunit 5. Robust succinic acid production by an engineered yeast Y. lipolytica from crude glycerol without pre-treatment was demonstrated. Diversion of metabolic flow from tricarboxylic acid cycle led to the success in generating a succinic acid producer Y. lipolytica PGC01003. The fermentation media and conditions were optimized, which resulted in 43 g L(-1) succinic acid production from crude glycerol. Using the fed-batch strategy in 2.5 L fermenter, up to 160 g L(-1) SA was yielded, indicating the great industrial potential. Inactivation of SDH5 in Y. lipolytica Po1f led to succinic acid accumulation and secretion significantly. To our best knowledge, this is the highest titer obtained in fermentation on succinic acid production. In addition, the performance of batch and fed-batch fermentation showed high tolerance and yield on biodiesel by-product crude glycerol. All these results indicated that PGC01003 is a promising microbial factorial cell for the highly efficient strategy solving the environmental problem in connection with the production of value-added product.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 100 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 100 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 19 19%
Researcher 19 19%
Student > Master 11 11%
Student > Doctoral Student 9 9%
Student > Bachelor 9 9%
Other 12 12%
Unknown 21 21%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 26 26%
Agricultural and Biological Sciences 17 17%
Chemical Engineering 9 9%
Engineering 9 9%
Environmental Science 4 4%
Other 8 8%
Unknown 27 27%

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 August 2020.
All research outputs
#5,807,148
of 18,476,586 outputs
Outputs from Biotechnology for Biofuels
#442
of 1,303 outputs
Outputs of similar age
#92,846
of 272,154 outputs
Outputs of similar age from Biotechnology for Biofuels
#2
of 6 outputs
Altmetric has tracked 18,476,586 research outputs across all sources so far. This one has received more attention than most of these and is in the 67th percentile.
So far Altmetric has tracked 1,303 research outputs from this source. They receive a mean Attention Score of 4.6. This one has gotten more attention than average, scoring higher than 64% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 272,154 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 64% of its contemporaries.
We're also able to compare this research output to 6 others from the same source and published within six weeks on either side of this one. This one has scored higher than 4 of them.