Title |
Establishment of an in vitro transcription system for Peste des petits ruminant virus
|
---|---|
Published in |
Virology Journal, December 2012
|
DOI | 10.1186/1743-422x-9-302 |
Pubmed ID | |
Authors |
Mohammad Yunus, Melkote S Shaila |
Abstract |
Peste-des-petits ruminants virus (PPRV) is a non segmented negative strand RNA virus of the genus Morbillivirus within Paramyxoviridae family. Negative strand RNA viruses are known to carry nucleocapsid (N) protein, phospho (P) protein and RNA polymerase (L protein) packaged within the virion which possess all activities required for transcription, post-transcriptional modification of mRNA and replication. In order to understand the mechanism of transcription and replication of the virus, an in vitro transcription reconstitution system is required. In the present work, an in vitro transcription system has been developed with ribonucleoprotein (RNP) complex purified from virus infected cells as well as partially purified recombinant polymerase (L-P) complex from insect cells along with N-RNA (genomic RNA encapsidated by N protein) template isolated from virus infected cells. |
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Demographic breakdown
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Science communicators (journalists, bloggers, editors) | 1 | 100% |
Mendeley readers
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Algeria | 1 | 5% |
Unknown | 20 | 95% |
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Student > Ph. D. Student | 5 | 24% |
Researcher | 3 | 14% |
Student > Bachelor | 2 | 10% |
Student > Postgraduate | 2 | 10% |
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Other | 2 | 10% |
Unknown | 5 | 24% |
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