Title |
The use of Loop-mediated Isothermal Amplification (LAMP) to detect the re-emerging Human African Trypanosomiasis (HAT) in the Luangwa and Zambezi valleys
|
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Published in |
Parasites & Vectors, December 2012
|
DOI | 10.1186/1756-3305-5-282 |
Pubmed ID | |
Authors |
Boniface Namangala, Lottie Hachaambwa, Kiichi Kajino, Aaron S Mweene, Kyouko Hayashida, Martin Simuunza, Humphrey Simukoko, Kennedy Choongo, Pamela Chansa, Shabir Lakhi, Ladslav Moonga, Amos Chota, Joseph Ndebe, Mutale Nsakashalo-Senkwe, Elizabeth Chizema, Lackson Kasonka, Chihiro Sugimoto |
Abstract |
Loop-mediated isothermal amplification (LAMP) is a novel strategy which amplifies DNA with high sensitivity and rapidity under isothermal conditions. In the present study, the performance of the repetitive insertion mobile element (RIME)-LAMP and human serum resistance-associated gene (SRA)-LAMP assays were evaluated using clinical specimens obtained from four male patients from Luangwa and Zambezi valleys in Zambia and Zimbabwe, respectively. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 1% |
Madagascar | 1 | 1% |
Brazil | 1 | 1% |
Unknown | 67 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 14 | 20% |
Student > Master | 12 | 17% |
Researcher | 10 | 14% |
Lecturer | 5 | 7% |
Student > Postgraduate | 4 | 6% |
Other | 11 | 16% |
Unknown | 14 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 18 | 26% |
Veterinary Science and Veterinary Medicine | 10 | 14% |
Biochemistry, Genetics and Molecular Biology | 8 | 11% |
Immunology and Microbiology | 7 | 10% |
Medicine and Dentistry | 6 | 9% |
Other | 4 | 6% |
Unknown | 17 | 24% |