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Multigene expression of protein complexes by iterative modification of genomic Bacmid DNA

Overview of attention for article published in BMC Molecular and Cell Biology, September 2009
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (87th percentile)
  • High Attention Score compared to outputs of the same age and source (92nd percentile)

Mentioned by

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1 X user
patent
3 patents

Citations

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29 Dimensions

Readers on

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71 Mendeley
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Title
Multigene expression of protein complexes by iterative modification of genomic Bacmid DNA
Published in
BMC Molecular and Cell Biology, September 2009
DOI 10.1186/1471-2199-10-87
Pubmed ID
Authors

Rob J Noad, Meredith Stewart, Mark Boyce, Cristina C Celma, Keith R Willison, Polly Roy

Abstract

Many cellular multi-protein complexes are naturally present in cells at low abundance. Baculovirus expression offers one approach to produce milligram quantities of correctly folded and processed eukaryotic protein complexes. However, current strategies suffer from the need to produce large transfer vectors, and the use of repeated promoter sequences in baculovirus, which itself produces proteins that promote homologous recombination. One possible solution to these problems is to construct baculovirus genomes that express each protein in a complex from a separate locus within the viral DNA. However current methods for selecting such recombinant genomes are too inefficient to routinely modify the virus in this way.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 71 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 3 4%
Italy 1 1%
Argentina 1 1%
Unknown 66 93%

Demographic breakdown

Readers by professional status Count As %
Researcher 24 34%
Student > Ph. D. Student 20 28%
Student > Master 9 13%
Student > Bachelor 5 7%
Professor 4 6%
Other 5 7%
Unknown 4 6%
Readers by discipline Count As %
Agricultural and Biological Sciences 38 54%
Biochemistry, Genetics and Molecular Biology 17 24%
Veterinary Science and Veterinary Medicine 3 4%
Immunology and Microbiology 3 4%
Medicine and Dentistry 2 3%
Other 2 3%
Unknown 6 8%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 August 2020.
All research outputs
#3,414,665
of 25,371,288 outputs
Outputs from BMC Molecular and Cell Biology
#51
of 1,233 outputs
Outputs of similar age
#11,617
of 102,308 outputs
Outputs of similar age from BMC Molecular and Cell Biology
#1
of 14 outputs
Altmetric has tracked 25,371,288 research outputs across all sources so far. Compared to these this one has done well and is in the 86th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,233 research outputs from this source. They receive a mean Attention Score of 4.0. This one has done particularly well, scoring higher than 95% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 102,308 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 87% of its contemporaries.
We're also able to compare this research output to 14 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 92% of its contemporaries.