Title |
Polyphasic characterization and genetic relatedness of low-virulence and virulent Listeria monocytogenes isolates
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Published in |
BMC Microbiology, December 2012
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DOI | 10.1186/1471-2180-12-304 |
Pubmed ID | |
Authors |
Sylvie M Roche, Olivier Grépinet, Annaëlle Kerouanton, Marie Ragon, Alexandre Leclercq, Stéphanie Témoin, Brigitte Schaeffer, Gilbert Skorski, Laurent Mereghetti, Alban Le Monnier, Philippe Velge |
Abstract |
Currently, food regulatory authorities consider all Listeria monocytogenes isolates as equally virulent. However, an increasing number of studies demonstrate extensive variations in virulence and pathogenicity of L. monocytogenes strains. Up to now, there is no comprehensive overview of the population genetic structure of L. monocytogenes taking into account virulence level. We have previously demonstrated that different low-virulence strains exhibit the same mutations in virulence genes suggesting that they could have common evolutionary pathways. New low-virulence strains were identified and assigned to phenotypic and genotypic Groups using cluster analysis. Pulsed-field gel electrophoresis, virulence gene sequencing and multi-locus sequence typing analyses were performed to study the genetic relatedness and the population structure between the studied low-virulence isolates and virulent strains. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Nigeria | 1 | 33% |
Spain | 1 | 33% |
Unknown | 1 | 33% |
Demographic breakdown
Type | Count | As % |
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Scientists | 2 | 67% |
Members of the public | 1 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Spain | 1 | 2% |
United States | 1 | 2% |
France | 1 | 2% |
Australia | 1 | 2% |
Unknown | 47 | 92% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 16 | 31% |
Student > Ph. D. Student | 15 | 29% |
Student > Master | 6 | 12% |
Student > Bachelor | 5 | 10% |
Student > Doctoral Student | 2 | 4% |
Other | 5 | 10% |
Unknown | 2 | 4% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 25 | 49% |
Biochemistry, Genetics and Molecular Biology | 7 | 14% |
Medicine and Dentistry | 6 | 12% |
Immunology and Microbiology | 5 | 10% |
Nursing and Health Professions | 2 | 4% |
Other | 3 | 6% |
Unknown | 3 | 6% |