Title |
Antisense-mediated isoform switching of steroid receptor coactivator-1 in the central nucleus of the amygdala of the mouse brain
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Published in |
BMC Neuroscience, January 2013
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DOI | 10.1186/1471-2202-14-5 |
Pubmed ID | |
Authors |
Ioannis Zalachoras, Gwendolynn Grootaers, Lisa TCM van Weert, Yves Aubert, Suzanne R de Kreij, Nicole A Datson, Willeke MC van Roon-Mom, Annemieke Aartsma-Rus, Onno C Meijer |
Abstract |
Antisense oligonucleotide (AON)-mediated exon skipping is a powerful tool to manipulate gene expression. In the present study we investigated the potential of exon skipping by local injection in the central nucleus of the amygdala (CeA) of the mouse brain. As proof of principle we targeted the splicing of steroid receptor coactivator-1 (SRC-1), a protein involved in nuclear receptor function. This nuclear receptor coregulator exists in two splice variants (SRC-1a and SRC-1e) which display differential distribution and opposing activities in the brain, and whose mRNAs differ in a single SRC-1e specific exon. |
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Demographic breakdown
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Mendeley readers
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Italy | 1 | 3% |
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Researcher | 12 | 36% |
Student > Master | 5 | 15% |
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Other | 4 | 12% |
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Medicine and Dentistry | 2 | 6% |
Other | 5 | 15% |
Unknown | 1 | 3% |