Title |
The modification and evaluation of an ELISA test for the surveillance of Mycobacterium avium subsp. paratuberculosisinfection in wild ruminants
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Published in |
BMC Veterinary Research, January 2013
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DOI | 10.1186/1746-6148-9-5 |
Pubmed ID | |
Authors |
Mathieu Pruvot, Taya L Forde, Jillian Steele, Susan J Kutz, Jeroen De Buck, Frank van der Meer, Karin Orsel |
Abstract |
Enzyme-linked immunosorbent assay (ELISA) is often used to test wildlife samples for Mycobacterium avium subsp. paratuberculosis (MAP) infection. However, commercially available kits are only validated for use with domestic ruminant species. A literature review was performed to document the current use of MAP serum ELISA in wild and semi-domestic ruminants. We then modified and evaluated a commercial ELISA kit (IDEXX Mycobacterium paratuberculosis Antibody Test Kit) for use with species for which it was not originally developed: elk (Cervus elaphus), bison (Bison bison) and caribou (Rangifer tarandus). We tested the affinity of different conjugates for immunoglobulin G (IgG) isolated from these species, performed checkerboard tests to determine the optimal dilutions of samples and conjugates, and established cut-off values using two different methods: a Receiver Operational Curve on a panel of known samples for elk, and an alternate method involving a panel of unknown serum samples for the three species. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Denmark | 1 | 2% |
Canada | 1 | 2% |
Unknown | 52 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 18 | 33% |
Student > Master | 8 | 15% |
Student > Bachelor | 6 | 11% |
Researcher | 5 | 9% |
Student > Postgraduate | 3 | 6% |
Other | 5 | 9% |
Unknown | 9 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 21 | 39% |
Veterinary Science and Veterinary Medicine | 13 | 24% |
Immunology and Microbiology | 3 | 6% |
Biochemistry, Genetics and Molecular Biology | 2 | 4% |
Social Sciences | 2 | 4% |
Other | 3 | 6% |
Unknown | 10 | 19% |