Title |
Optimizing hybrid assembly of next-generation sequence data from Enterococcus faecium: a microbe with highly divergent genome
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Published in |
BMC Systems Biology, December 2012
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DOI | 10.1186/1752-0509-6-s3-s21 |
Pubmed ID | |
Authors |
Yajun Wang, Yao Yu, Bohu Pan, Pei Hao, Yixue Li, Zhifeng Shao, Xiaogang Xu, Xuan Li |
Abstract |
Sequencing of bacterial genomes became an essential approach to study pathogen virulence and the phylogenetic relationship among close related strains. Bacterium Enterococcus faecium emerged as an important nosocomial pathogen that were often associated with resistance to common antibiotics in hospitals. With highly divergent gene contents, it presented a challenge to the next generation sequencing (NGS) technologies featuring high-throughput and shorter read-length. This study was designed to investigate the properties and systematic biases of NGS technologies and evaluate critical parameters influencing the outcomes of hybrid assemblies using combinations of NGS data. |
Mendeley readers
Geographical breakdown
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Mexico | 1 | 2% |
Croatia | 1 | 2% |
Unknown | 49 | 91% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 14 | 26% |
Student > Ph. D. Student | 13 | 24% |
Student > Master | 6 | 11% |
Student > Bachelor | 3 | 6% |
Professor | 3 | 6% |
Other | 10 | 19% |
Unknown | 5 | 9% |
Readers by discipline | Count | As % |
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Computer Science | 3 | 6% |
Veterinary Science and Veterinary Medicine | 2 | 4% |
Other | 4 | 7% |
Unknown | 5 | 9% |