Title |
Primer development to obtain complete coding sequence of HA and NA genes of influenza A/H3N2 virus
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Published in |
BMC Research Notes, August 2016
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DOI | 10.1186/s13104-016-2235-8 |
Pubmed ID | |
Authors |
Agustiningsih Agustiningsih, Hidayat Trimarsanto, Vivi Setiawaty, I. Made Artika, David Handojo Muljono |
Abstract |
Influenza is an acute respiratory illness and has become a serious public health problem worldwide. The need to study the HA and NA genes in influenza A virus is essential since these genes frequently undergo mutations. This study describes the development of primer sets for RT-PCR to obtain complete coding sequence of Hemagglutinin (HA) and Neuraminidase (NA) genes of influenza A/H3N2 virus from Indonesia. The primers were developed based on influenza A/H3N2 sequence worldwide from Global Initiative on Sharing All Influenza Data (GISAID) and further tested using Indonesian influenza A/H3N2 archived samples of influenza-like illness (ILI) surveillance from 2008 to 2009. An optimum RT-PCR condition was acquired for all HA and NA fragments designed to cover complete coding sequence of HA and NA genes. A total of 71 samples were successfully sequenced for complete coding sequence both of HA and NA genes out of 145 samples of influenza A/H3N2 tested. The developed primer sets were suitable for obtaining complete coding sequences of HA and NA genes of Indonesian samples from 2008 to 2009. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 44 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 8 | 18% |
Student > Master | 5 | 11% |
Researcher | 4 | 9% |
Lecturer | 4 | 9% |
Professor > Associate Professor | 3 | 7% |
Other | 10 | 23% |
Unknown | 10 | 23% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 8 | 18% |
Nursing and Health Professions | 5 | 11% |
Agricultural and Biological Sciences | 4 | 9% |
Medicine and Dentistry | 3 | 7% |
Unspecified | 2 | 5% |
Other | 10 | 23% |
Unknown | 12 | 27% |