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Quantification of anandamide content in animal cells and tissues: the normalization makes the difference

Overview of attention for article published in Lipids in Health and Disease, November 2002
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Title
Quantification of anandamide content in animal cells and tissues: the normalization makes the difference
Published in
Lipids in Health and Disease, November 2002
DOI 10.1186/1476-511x-1-4
Pubmed ID
Authors

Mauro Maccarrone, Monica Bari, Alessandro Finazzi-Agrò

Abstract

Anandamide (N-arachidonoylethanolamine, AEA) is an endogenous lipid that binds to cannabinoid receptors in the central nervous system and in peripheral cells. Quantitative analysis of AEA is generally based on the normalization to the fresh weight of the samples. Here, we show that the normalization procedure of AEA content is such a critical factor, that it might introduce per se significant discrepancies in the quantification of AEA even in the same sample. We suggest that a rapid, accurate and most reliable reference to quantify AEA and congeners from different sources is the protein content, a common parameter to cells and tissues.

Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 November 2014.
All research outputs
#22,759,452
of 25,374,647 outputs
Outputs from Lipids in Health and Disease
#1,336
of 1,608 outputs
Outputs of similar age
#132,660
of 135,027 outputs
Outputs of similar age from Lipids in Health and Disease
#2
of 3 outputs
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