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PCR detection of Burkholderia multivorans in water and soil samples

Overview of attention for article published in BMC Microbiology, August 2016
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Title
PCR detection of Burkholderia multivorans in water and soil samples
Published in
BMC Microbiology, August 2016
DOI 10.1186/s12866-016-0801-9
Pubmed ID
Authors

Charlotte Peeters, Stijn Daenekindt, Peter Vandamme

Abstract

Although semi-selective growth media have been developed for the isolation of Burkholderia cepacia complex bacteria from the environment, thus far Burkholderia multivorans has rarely been isolated from such samples. Because environmental B. multivorans isolates mainly originate from water samples, we hypothesized that water rather than soil is its most likely environmental niche. The aim of the present study was to assess the occurrence of B. multivorans in water samples from Flanders (Belgium) using a fast, culture-independent PCR assay. A nested PCR approach was used to achieve high sensitivity, and specificity was confirmed by sequencing the resulting amplicons. B. multivorans was detected in 11 % of the water samples (n = 112) and 92 % of the soil samples (n = 25) tested. The percentage of false positives was higher for water samples compared to soil samples, showing that the presently available B. multivorans recA primers lack specificity when applied to the analysis of water samples. The results of the present study demonstrate that B. multivorans DNA is commonly present in soil samples and to a lesser extent in water samples in Flanders (Belgium).

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 29 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 29 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 7 24%
Student > Master 5 17%
Student > Ph. D. Student 3 10%
Student > Bachelor 2 7%
Other 2 7%
Other 2 7%
Unknown 8 28%
Readers by discipline Count As %
Agricultural and Biological Sciences 9 31%
Biochemistry, Genetics and Molecular Biology 5 17%
Pharmacology, Toxicology and Pharmaceutical Science 2 7%
Social Sciences 2 7%
Nursing and Health Professions 1 3%
Other 2 7%
Unknown 8 28%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 September 2016.
All research outputs
#20,340,423
of 22,886,568 outputs
Outputs from BMC Microbiology
#2,695
of 3,196 outputs
Outputs of similar age
#311,462
of 355,880 outputs
Outputs of similar age from BMC Microbiology
#72
of 85 outputs
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So far Altmetric has tracked 3,196 research outputs from this source. They receive a mean Attention Score of 4.1. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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