Title |
Classical swine fever virus replicated poorly in cells from MxA transgenic pigs
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Published in |
BMC Veterinary Research, August 2016
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DOI | 10.1186/s12917-016-0794-5 |
Pubmed ID | |
Authors |
Yicheng Zhao, Tiedong Wang, Li Yao, Bo Liu, Chunbo Teng, Hongsheng Ouyang |
Abstract |
In addition to their value as livestock, pigs are susceptible to classical swine fever virus (CSFV) and can serve as reservoirs for CSFV, allowing it to develop into an epizootic. CSFV, a pestivirus of the Flaviviridae family, has a single-stranded RNA genome. Recent research has indicated that the human MxA protein inhibits the life cycles of certain RNA viruses, such as members of the Bunyaviridae family, the Flaviviridae family and others. To produce pigs with antiviral protection against CSFV, transgenic pigs expressing human MxA were generated by nuclear transplantation. Cells from three MxA transgenic piglets were used to investigate in vitro antiviral activity of MxA aganist CSFV, and the results of in vitro indirect immunofluorescence assays, virus titration and real-time PCR indicated that the MxA transgenic pig has an antiviral capacity against CSFV. Transgene with human MxA on pigs is feasible. High levels of MxA expression do inhibit CSFV in vitro at early time points post-infection at 60-96dpi. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 19 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 5 | 26% |
Student > Bachelor | 3 | 16% |
Researcher | 2 | 11% |
Lecturer | 1 | 5% |
Lecturer > Senior Lecturer | 1 | 5% |
Other | 2 | 11% |
Unknown | 5 | 26% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 5 | 26% |
Veterinary Science and Veterinary Medicine | 4 | 21% |
Agricultural and Biological Sciences | 2 | 11% |
Immunology and Microbiology | 2 | 11% |
Social Sciences | 1 | 5% |
Other | 0 | 0% |
Unknown | 5 | 26% |