Title |
Whole genome sequence analysis indicates recent diversification of mammal-associated Campylobacter fetus and implicates a genetic factor associated with H2S production
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Published in |
BMC Genomics, September 2016
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DOI | 10.1186/s12864-016-3058-7 |
Pubmed ID | |
Authors |
Linda van der Graaf–van Bloois, Birgitta Duim, William G. Miller, Ken J. Forbes, Jaap A. Wagenaar, Aldert Zomer |
Abstract |
Campylobacter fetus (C. fetus) can cause disease in both humans and animals. C. fetus has been divided into three subspecies: C. fetus subsp. fetus (Cff), C. fetus subsp. venerealis (Cfv) and C. fetus subsp. testudinum (Cft). Subspecies identification of mammal-associated C. fetus strains is crucial in the control of Bovine Genital Campylobacteriosis (BGC), a syndrome associated with Cfv. The prescribed methods for subspecies identification of the Cff and Cfv isolates are: tolerance to 1 % glycine and H2S production. In this study, we observed the deletion of a putative cysteine transporter in the Cfv strains, which are not able to produce H2S from L-cysteine. Phylogenetic reconstruction of the core genome single nucleotide polymorphisms (SNPs) within Cff and Cfv strains divided these strains into five different clades and showed that the Cfv clade and a Cff clade evolved from a single Cff ancestor. Multiple C. fetus clades were observed, which were not consistent with the biochemical differentiation of the strains. This suggests the need for a closer evaluation of the current C. fetus subspecies differentiation, considering that the phenotypic differentiation is still applied in BGC control programs. |
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Scientists | 2 | 67% |
Members of the public | 1 | 33% |
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Geographical breakdown
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Student > Bachelor | 6 | 16% |
Researcher | 4 | 11% |
Student > Master | 3 | 8% |
Professor > Associate Professor | 2 | 5% |
Other | 5 | 14% |
Unknown | 8 | 22% |
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Pharmacology, Toxicology and Pharmaceutical Science | 1 | 3% |
Other | 4 | 11% |
Unknown | 8 | 22% |