Title |
Analysis of strain relatedness using High Resolution Melting in a case of recurrent candiduria
|
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Published in |
BMC Microbiology, January 2013
|
DOI | 10.1186/1471-2180-13-13 |
Pubmed ID | |
Authors |
Sara Gago, Belen Lorenzo, Alicia Gomez-Lopez, Isabel Cuesta, Manuel Cuenca-Estrella, Maria J Buitrago |
Abstract |
Several genotyping protocols have been described to study Candida albicans strains with different sensitivity values. In this study we have analyzed the genetic relatedness and the antifungal susceptibility of several Candida albicans strains isolated from a patient who from suffered recurrent candiduria for a period of five years. Strains were genotyped using Microsatellite Length Polymorphism (MLP) with three microsatellite markers (HIS 3, EF 3 and CDC 3), and a new method based on high resolution melting (HRM) was developed to analyze the microsatellite region. This method was compared with the conventional technique that uses capillary electrophoresis. |
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Mendeley readers
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