Title |
Long non-coding RNA XIST regulates gastric cancer progression by acting as a molecular sponge of miR-101 to modulate EZH2 expression
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Published in |
Journal of Experimental & Clinical Cancer Research, September 2016
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DOI | 10.1186/s13046-016-0420-1 |
Pubmed ID | |
Authors |
Dong-liang Chen, Huai-qiang Ju, Yun-xin Lu, Le-zong Chen, Zhao-lei Zeng, Dong-sheng Zhang, Hui-yan Luo, Feng Wang, Miao-zhen Qiu, De-shen Wang, Da-zhi Xu, Zhi-wei Zhou, Helene Pelicano, Peng Huang, Dan Xie, Feng-hua Wang, Yu-hong Li, Rui-hua Xu |
Abstract |
Long non-coding RNAs (lncRNAs) have emerged as critical regulators of tumor progression. However, the role and molecular mechanism of lncRNA XIST in gastric cancer is still unknown. Real-time PCR analysis was performed to measure the expression levels of lncRNA XIST in gastric cancer tissues and cell lines, the correlation between lncRNA XIST expression and clinicopathological characteristics and prognosis was analyzed in gastric cancer patients. The biological function of lncRNA XIST on gastric cancer cells were determined both in vitro and in vivo. The regulating relationship between lncRNA XIST and miR-101 was investigated in gastric cancer cells. lncRNA XIST was significantly up-regulated in gastric cancer tissues and cell lines. Overexpression of lncRNA XIST was markedly associated with larger tumor size, lymph node invasion, distant metastasis and TNM stage in gastric cancer patients. Functionally, knockdown of lncRNA XIST exerted tumor-suppressive effects by inhibiting cell proliferation, migration and invasion in vitro and tumor growth and metastasis in vivo. Furthermore, an inverse relationship between lncRNA XIST and miR-101 was found. Polycomb group protein enhancer of zeste homolog 2 (EZH2), a direct target of miR-101, could mediated the biological effects that lncRNA XIST exerted. lncRNA XIST is up-regulated and is associated with aggressive tumor phenotypes and patient survival in gastric cancer, and the newly identified lncRNA XIST/miR-101/EZH2 axis could be a potential biomarkers or therapeutic targets for gastric cancer patients. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 52 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 12 | 23% |
Student > Master | 8 | 15% |
Professor | 5 | 10% |
Researcher | 4 | 8% |
Student > Bachelor | 3 | 6% |
Other | 4 | 8% |
Unknown | 16 | 31% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 15 | 29% |
Medicine and Dentistry | 8 | 15% |
Agricultural and Biological Sciences | 3 | 6% |
Immunology and Microbiology | 2 | 4% |
Psychology | 1 | 2% |
Other | 3 | 6% |
Unknown | 20 | 38% |