Title |
Differences in sequencing technologies improve the retrieval of anammox bacterial genome from metagenomes
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Published in |
BMC Genomics, January 2013
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DOI | 10.1186/1471-2164-14-7 |
Pubmed ID | |
Authors |
Fabio Gori, Susannah G Tringe, Gianluigi Folino, Sacha AFT van Hijum, Huub JM Op den Camp, Mike SM Jetten, Elena Marchiori |
Abstract |
Sequencing technologies have different biases, in single-genome sequencing and metagenomic sequencing; these can significantly affect ORFs recovery and the population distribution of a metagenome. In this paper we investigate how well different technologies represent information related to a considered organism of interest in a metagenome, and whether it is beneficial to combine information obtained using different technologies. We analyze comparatively three metagenomic datasets acquired from a sample containing the anammox bacterium Candidatus 'Brocadia fulgida' (B. fulgida). These datasets were obtained using Roche 454 FLX and Sanger sequencing with two different libraries (shotgun and fosmid). |
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United States | 1 | 100% |
Demographic breakdown
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
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United States | 2 | 4% |
Netherlands | 2 | 4% |
Canada | 1 | 2% |
Unknown | 41 | 89% |
Demographic breakdown
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Student > Master | 12 | 26% |
Researcher | 11 | 24% |
Student > Ph. D. Student | 8 | 17% |
Professor | 3 | 7% |
Student > Bachelor | 2 | 4% |
Other | 5 | 11% |
Unknown | 5 | 11% |
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Environmental Science | 9 | 20% |
Engineering | 2 | 4% |
Immunology and Microbiology | 1 | 2% |
Biochemistry, Genetics and Molecular Biology | 1 | 2% |
Other | 0 | 0% |
Unknown | 8 | 17% |