Complete genome sequence of Corynebacterium pseudotuberculosis biovar ovis strain P54B96 isolated from antelope in South Africa obtained by rapid next generation sequencing technology

Syed Shah Hassan, Luis Carlos Guimarães, Ulisses de Pádua Pereira, Arshad Islam, Amjad Ali, Syeda Marriam Bakhtiar, Dayana Ribeiro, Anderson Rodrigues dos Santos, Siomar de Castro Soares, Fernanda Dorella, Anne Cybelle Pinto, Maria Paula Cruz Schneider, Maria Silvanira Barbosa, Síntia Almeida, Vinícius Abreu, Flávia Aburjaile, Adriana Ribeiro Carneiro, Louise Teixeira Cerdeira, Karina Fiaux, Eudes Barbosa, Carlos Diniz, Flavia S. Rocha, Rommel Thiago Jucá Ramos, Neha Jain, Sandeep Tiwari, Debmalya Barh, Anderson Miyoshi, Borna Müller, Artur Silva, Vasco Azevedo

The Actinobacteria, Corynebacterium pseudotuberculosis strain P54B96, a nonmotile, non-sporulating and a mesophile bacterium, was isolated from liver, lung and mediastinal lymph node lesions in an antelope from South Africa. This strain is interesting in the sense that it has been found together with non-tuberculous mycobacteria (NTMs) which could nevertheless play a role in the lesion formation. In this work, we describe a set of features of C. pseudotuberculosis P54B96, together with the details of the complete genome sequence and annotation. The genome comprises of 2.34 Mbp long, single circular genome with 2,084 protein-coding genes, 12 rRNA, 49 tRNA and 62 pseudogenes and a G+C content of 52.19%. The analysis of the genome sequence provides means to better understanding the molecular and genetic basis of virulence of this bacterium, enabling a detailed investigation of its pathogenesis.