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Mendeley readers
Attention Score in Context
| Title |
A novel expression system for production of soluble prion proteins in E. coli
|
|---|---|
| Published in |
Microbial Cell Factories, January 2012
|
| DOI | 10.1186/1475-2859-11-6 |
| Pubmed ID | |
| Authors |
Romany NN Abskharon, Stephanie Ramboarina, Hassan El Hassan, Wael Gad, Marcin I Apostol, Gabriele Giachin, Giuseppe Legname, Jan Steyaert, Joris Messens, Sameh H Soror, Alexandre Wohlkonig |
| Abstract |
Expression of eukaryotic proteins in Escherichia coli is challenging, especially when they contain disulfide bonds. Since the discovery of the prion protein (PrP) and its role in transmissible spongiform encephalopathies, the need to obtain large quantities of the recombinant protein for research purposes has been essential. Currently, production of recombinant PrP is achieved by refolding protocols. Here, we show that the co-expression of two different PrP with the human Quiescin Sulfhydryl OXidase (QSOX), a human chaperone with thiol/disulfide oxidase activity, in the cytoplasm of E. coli produces soluble recombinant PrP. The structural integrity of the soluble PrP has been confirmed by nuclear magnetic resonance spectroscopy, demonstrating that properly folded PrP can be easily expressed in bacteria. Furthermore, the soluble recombinant PrP produced with this method can be used for functional and structural studies. |
Mendeley readers
The data shown below were compiled from readership statistics for 78 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 1% |
| Mexico | 1 | 1% |
| India | 1 | 1% |
| Greece | 1 | 1% |
| Unknown | 74 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 19 | 24% |
| Researcher | 16 | 21% |
| Student > Bachelor | 11 | 14% |
| Student > Master | 6 | 8% |
| Student > Postgraduate | 5 | 6% |
| Other | 9 | 12% |
| Unknown | 12 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 33 | 42% |
| Biochemistry, Genetics and Molecular Biology | 22 | 28% |
| Chemistry | 5 | 6% |
| Neuroscience | 2 | 3% |
| Immunology and Microbiology | 2 | 3% |
| Other | 0 | 0% |
| Unknown | 14 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 January 2013.
All research outputs
#20,182,546
of 22,696,971 outputs
Outputs from Microbial Cell Factories
#1,350
of 1,584 outputs
Outputs of similar age
#220,340
of 243,325 outputs
Outputs of similar age from Microbial Cell Factories
#35
of 35 outputs
Altmetric has tracked 22,696,971 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,584 research outputs from this source. They receive a mean Attention Score of 4.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 243,325 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 35 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.