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The dual effect of a ferredoxin-hydrogenase fusion protein in vivo: successful divergence of the photosynthetic electron flux towards hydrogen production and elevated oxygen tolerance

Overview of attention for article published in Biotechnology for Biofuels, August 2016
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • One of the highest-scoring outputs from this source (#10 of 1,230)
  • High Attention Score compared to outputs of the same age (95th percentile)
  • High Attention Score compared to outputs of the same age and source (99th percentile)

Mentioned by

news
6 news outlets
twitter
3 tweeters
wikipedia
1 Wikipedia page

Citations

dimensions_citation
48 Dimensions

Readers on

mendeley
77 Mendeley
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Title
The dual effect of a ferredoxin-hydrogenase fusion protein in vivo: successful divergence of the photosynthetic electron flux towards hydrogen production and elevated oxygen tolerance
Published in
Biotechnology for Biofuels, August 2016
DOI 10.1186/s13068-016-0601-3
Pubmed ID
Authors

Haviva Eilenberg, Iddo Weiner, Oren Ben-Zvi, Carmel Pundak, Abigail Marmari, Oded Liran, Matt S. Wecker, Yuval Milrad, Iftach Yacoby

Abstract

Hydrogen photo-production in green algae, catalyzed by the enzyme [FeFe]-hydrogenase (HydA), is considered a promising source of renewable clean energy. Yet, a significant increase in hydrogen production efficiency is necessary for industrial scale-up. We have previously shown that a major challenge to be resolved is the inferior competitiveness of HydA with NADPH production, catalyzed by ferredoxin-NADP(+)-reductase (FNR). In this work, we explored the in vivo hydrogen production efficiency of Fd-HydA, where the electron donor ferredoxin (Fd) is fused to HydA and expressed in the model organism Chlamydomonas reinhardtii. We show that once the Fd-HydA fusion gene is expressed in micro-algal cells of C. reinhardtii, the fusion enzyme is able to intercept photosynthetic electrons and use them for efficient hydrogen production, thus supporting the previous observations made in vitro. We found that Fd-HydA has a ~4.5-fold greater photosynthetic hydrogen production rate standardized for hydrogenase amount (PHPRH) than that of the native HydA in vivo. Furthermore, we provide evidence suggesting that the fusion protein is more resistant to oxygen than the native HydA. The in vivo photosynthetic activity of the Fd-HydA enzyme surpasses that of the native HydA and shows higher oxygen tolerance. Therefore, our results provide a solid platform for further engineering efforts towards efficient hydrogen production in microalgae through the expression of synthetic enzymes.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 77 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 1 1%
Argentina 1 1%
Unknown 75 97%

Demographic breakdown

Readers by professional status Count As %
Student > Master 21 27%
Student > Ph. D. Student 17 22%
Student > Bachelor 11 14%
Professor 7 9%
Researcher 6 8%
Other 8 10%
Unknown 7 9%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 31 40%
Agricultural and Biological Sciences 19 25%
Engineering 6 8%
Chemical Engineering 3 4%
Environmental Science 3 4%
Other 6 8%
Unknown 9 12%

Attention Score in Context

This research output has an Altmetric Attention Score of 54. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 March 2020.
All research outputs
#461,552
of 17,175,498 outputs
Outputs from Biotechnology for Biofuels
#10
of 1,230 outputs
Outputs of similar age
#12,621
of 276,627 outputs
Outputs of similar age from Biotechnology for Biofuels
#1
of 11 outputs
Altmetric has tracked 17,175,498 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 97th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,230 research outputs from this source. They receive a mean Attention Score of 4.5. This one has done particularly well, scoring higher than 99% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 276,627 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 95% of its contemporaries.
We're also able to compare this research output to 11 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 99% of its contemporaries.