Title |
Silver nanoparticles of Albizia adianthifolia: the induction of apoptosis in human lung carcinoma cell line
|
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Published in |
Journal of Nanobiotechnology, February 2013
|
DOI | 10.1186/1477-3155-11-5 |
Pubmed ID | |
Authors |
Rishalan Govender, Alisa Phulukdaree, Robert M Gengan, Krishnan Anand, Anil A Chuturgoon |
Abstract |
Silver nanoparticles (AgNP), the most popular nano-compounds, possess unique properties. Albizia adianthifolia (AA) is a plant of the Fabaceae family that is rich in saponins. The biological properties of a novel AgNP, synthesized from an aqueous leaf extract of AA (AA(AgNP)), were investigated on A549 lung cells. Cell viability was determined by the MTT assay. Cellular oxidative status (lipid peroxidation and glutathione (GSH) levels), ATP concentration, caspase-3/-7, -8 and -9 activities were determined. Apoptosis, mitochondrial (mt) membrane depolarization (flow cytometry) and DNA fragmentation (comet assay) were assessed. The expression of CD95 receptors, p53, bax, PARP-1 and smac/DIABLO was evaluated by flow cytometry and/or western blotting. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Nepal | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | <1% |
United States | 1 | <1% |
Russia | 1 | <1% |
Canada | 1 | <1% |
Unknown | 115 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 31 | 26% |
Researcher | 16 | 13% |
Student > Master | 16 | 13% |
Student > Bachelor | 11 | 9% |
Professor > Associate Professor | 5 | 4% |
Other | 16 | 13% |
Unknown | 24 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 22 | 18% |
Biochemistry, Genetics and Molecular Biology | 21 | 18% |
Chemistry | 13 | 11% |
Medicine and Dentistry | 8 | 7% |
Pharmacology, Toxicology and Pharmaceutical Science | 5 | 4% |
Other | 18 | 15% |
Unknown | 32 | 27% |