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Attention Score in Context
| Title |
In vivo whole-cell recording with high success rate in anaesthetized and awake mammalian brains
|
|---|---|
| Published in |
Molecular Brain, September 2016
|
| DOI | 10.1186/s13041-016-0266-7 |
| Pubmed ID | |
| Authors |
Yao Wang, Yu-zhang Liu, Shi-yi Wang, Zhiru Wang |
| Abstract |
As a critical technique for dissection of synaptic and cellular mechanisms, whole-cell patch-clamp recording has become feasible for in vivo preparations including both anaesthetized and awake mammalian brains. However, compared with in vitro whole-cell recording, in vivo whole-cell recording often suffers from low success rates and high access resistance, preventing its wide application in physiological analysis of neural circuits. Here, we describe experimental procedures for achieving in vivo amphotericin B-perforated whole-cell recording as well as conventional (breakthrough) whole-cell recording from rats and mice. The success rate of perforated whole-cell recordings was 70-80 % in the hippocampus and neocortex, and access resistance was 40-70 MΩ. The success rate of conventional whole-cell recordings was ~50 % in the hippocampus, with access resistance of 20-40 MΩ. Recordings were stable, and in awake, head-fixed animals, ~50 % whole-cell patched neurons could be held for > 1 hr. The conventional whole-cell recording also permitted infusion of pharmacological agents, such as intracellular blockers of Na(+) channels and NMDA receptors. These findings open new possibilities for synaptic and cellular analysis in vivo. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Science communicators (journalists, bloggers, editors) | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 89 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 1% |
| Unknown | 88 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 22 | 25% |
| Student > Master | 15 | 17% |
| Researcher | 14 | 16% |
| Student > Postgraduate | 6 | 7% |
| Student > Bachelor | 5 | 6% |
| Other | 10 | 11% |
| Unknown | 17 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Neuroscience | 34 | 38% |
| Agricultural and Biological Sciences | 13 | 15% |
| Biochemistry, Genetics and Molecular Biology | 7 | 8% |
| Engineering | 5 | 6% |
| Medicine and Dentistry | 5 | 6% |
| Other | 8 | 9% |
| Unknown | 17 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 October 2016.
All research outputs
#20,656,820
of 25,374,647 outputs
Outputs from Molecular Brain
#924
of 1,198 outputs
Outputs of similar age
#255,811
of 330,424 outputs
Outputs of similar age from Molecular Brain
#13
of 15 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 10th percentile – i.e., 10% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,198 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.9. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 330,424 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 12th percentile – i.e., 12% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 15 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.