Title |
Effects of the breed, sex and age on cellular content and growth factor release from equine pure-platelet rich plasma and pure-platelet rich gel
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Published in |
BMC Veterinary Research, February 2013
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DOI | 10.1186/1746-6148-9-29 |
Pubmed ID | |
Authors |
Carlos E Giraldo, Catalina López, María E Álvarez, Ismael J Samudio, Marta Prades, Jorge U Carmona |
Abstract |
There is no information on the effects of the breed, gender and age on the cellular content and growth factor (GF) release from equine pure-platelet rich plasma (P-PRP) and pure-platelet rich gel (P-PRG). The objectives of this study were: 1) to compare the cellular composition of P-PRP with whole blood and platelet poor plasma (PPP); 2) to compare the concentration of transforming GF beta 1 (TGF-β1) and platelet derived GF isoform BB (PDGF-BB) between P-PRP treated with non-ionic detergent (P-PRP+NID), P-PRG (activated with calcium gluconate -CG-), PPP+NID, PPP gel (PPG), and plasma and; 3) to evaluate and to correlate the effect of the breed, gender and age on the cellular and GF concentration for each blood component. Forty adult horses, 20 Argentinean Creole Horses (ACH) and, 20 Colombian Creole Horses (CCH) were included. Data were analyzed by parametric (i.e.: t-test, one way ANOVA) and non parametric (Kruskal-Wallis test, Wilcoxon test) tests. Correlation analysis was also performed by using the Spearman and Pearson tests. A p ≤ 0.05 was set as significant for all tests. All the blood components were compared for platelet (PLT), leukocyte (WBC), TGF-β1 and PDGF-BB concentrations. The effect of the breed, gender and age on these variables was analyzed. A P ≤ 0.05 was accepted as significant for all the tests. |
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Geographical breakdown
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Colombia | 1 | 1% |
Italy | 1 | 1% |
Peru | 1 | 1% |
Canada | 1 | 1% |
Unknown | 88 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 17 | 18% |
Student > Bachelor | 14 | 15% |
Researcher | 9 | 10% |
Student > Postgraduate | 9 | 10% |
Student > Doctoral Student | 5 | 5% |
Other | 17 | 18% |
Unknown | 21 | 23% |
Readers by discipline | Count | As % |
---|---|---|
Veterinary Science and Veterinary Medicine | 26 | 28% |
Medicine and Dentistry | 24 | 26% |
Agricultural and Biological Sciences | 7 | 8% |
Biochemistry, Genetics and Molecular Biology | 3 | 3% |
Engineering | 2 | 2% |
Other | 4 | 4% |
Unknown | 26 | 28% |