Title |
Glucose-methanol co-utilization in Pichia pastoris studied by metabolomics and instationary 13C flux analysis
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Published in |
BMC Systems Biology, February 2013
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DOI | 10.1186/1752-0509-7-17 |
Pubmed ID | |
Authors |
Joel Jordà, Camilo Suarez, Marc Carnicer, Angela ten Pierick, Joseph J Heijnen, Walter van Gulik, Pau Ferrer, Joan Albiol, Aljoscha Wahl |
Abstract |
Several studies have shown that the utilization of mixed carbon feeds instead of methanol as sole carbon source is beneficial for protein production with the methylotrophic yeast Pichia pastoris. In particular, growth under mixed feed conditions appears to alleviate the metabolic burden related to stress responses triggered by protein overproduction and secretion. Yet, detailed analysis of the metabolome and fluxome under mixed carbon source metabolizing conditions are missing. To obtain a detailed flux distribution of central carbon metabolism, including the pentose phosphate pathway under methanol-glucose conditions, we have applied metabolomics and instationary ¹³C flux analysis in chemostat cultivations. |
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Geographical breakdown
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United States | 1 | 100% |
Demographic breakdown
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Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
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United States | 3 | 2% |
Germany | 2 | 1% |
Chile | 1 | <1% |
Austria | 1 | <1% |
Brazil | 1 | <1% |
Portugal | 1 | <1% |
United Kingdom | 1 | <1% |
Sweden | 1 | <1% |
Spain | 1 | <1% |
Other | 1 | <1% |
Unknown | 158 | 92% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 52 | 30% |
Researcher | 26 | 15% |
Student > Master | 18 | 11% |
Other | 11 | 6% |
Student > Bachelor | 10 | 6% |
Other | 31 | 18% |
Unknown | 23 | 13% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 59 | 35% |
Biochemistry, Genetics and Molecular Biology | 37 | 22% |
Engineering | 23 | 13% |
Chemical Engineering | 8 | 5% |
Chemistry | 8 | 5% |
Other | 9 | 5% |
Unknown | 27 | 16% |