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Short-term culture of ovarian cortex pieces to assess the cryopreservation outcome in wild felids for genome conservation

Overview of attention for article published in BMC Veterinary Research, February 2013
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • Among the highest-scoring outputs from this source (#38 of 3,297)
  • High Attention Score compared to outputs of the same age (97th percentile)
  • High Attention Score compared to outputs of the same age and source (97th percentile)

Mentioned by

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6 news outlets
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3 X users

Citations

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24 Dimensions

Readers on

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47 Mendeley
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Title
Short-term culture of ovarian cortex pieces to assess the cryopreservation outcome in wild felids for genome conservation
Published in
BMC Veterinary Research, February 2013
DOI 10.1186/1746-6148-9-37
Pubmed ID
Authors

Caterina Wiedemann, Jennifer Zahmel, Katarina Jewgenow

Abstract

Cryopreservation of ovarian tissue has the potential to preserve female germ cells of endangered mammals. In the present study, a freezing protocol successfully used for human tissue, was adapted for preserving ovarian tissue of domestic and non-domestic felids. Ovaries from non-domestic felid species were obtained from seven freshly euthanized and two recently deceased wild felids kept in different European Zoos. In addition, ovaries from domestic cats were obtained after ovariectomy from local veterinary clinics for methological adaptations.Ovarian cortex was dissected and uniform sized pieces of 2 mm diameter were obtained. Using a slow freezing protocol (-0.3°C per min) in 1.5 mol/L ethylene glycol, 0.1 mol/L sucrose, the pieces were cultured for up to 14 days both before and after cryopreservation. The integrity of primordial follicles was assessed by histology, and the impact of different protein sources (FCS or BSA) and Vitamin C was determined during two weeks of culture.

X Demographics

X Demographics

The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 47 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 47 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 13 28%
Student > Master 7 15%
Student > Bachelor 6 13%
Researcher 6 13%
Student > Doctoral Student 4 9%
Other 6 13%
Unknown 5 11%
Readers by discipline Count As %
Agricultural and Biological Sciences 19 40%
Medicine and Dentistry 7 15%
Biochemistry, Genetics and Molecular Biology 6 13%
Veterinary Science and Veterinary Medicine 6 13%
Immunology and Microbiology 1 2%
Other 0 0%
Unknown 8 17%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 51. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 July 2017.
All research outputs
#821,123
of 25,371,288 outputs
Outputs from BMC Veterinary Research
#38
of 3,297 outputs
Outputs of similar age
#5,524
of 205,101 outputs
Outputs of similar age from BMC Veterinary Research
#1
of 44 outputs
Altmetric has tracked 25,371,288 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 96th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,297 research outputs from this source. They receive a mean Attention Score of 4.3. This one has done particularly well, scoring higher than 98% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 205,101 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 97% of its contemporaries.
We're also able to compare this research output to 44 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 97% of its contemporaries.