↓ Skip to main content

Specific humoral and cellular immunity induced by Trypanosoma cruzi DNA immunization in a canine model

Overview of attention for article published in Veterinary Research, March 2013
Altmetric Badge

Mentioned by

twitter
1 tweeter

Citations

dimensions_citation
19 Dimensions

Readers on

mendeley
52 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Specific humoral and cellular immunity induced by Trypanosoma cruzi DNA immunization in a canine model
Published in
Veterinary Research, March 2013
DOI 10.1186/1297-9716-44-15
Pubmed ID
Authors

Minerva Arce-Fonseca, Martha A Ballinas-Verdugo, Emma R Abreu Zenteno, Davinia Suárez-Flores, Silvia C Carrillo-Sánchez, Ricardo Alejandre-Aguilar, José Luis Rosales-Encina, Pedro A Reyes, Olivia Rodríguez-Morales

Abstract

Chagas disease has a high incidence in Mexico and other Latin American countries. Because one of the most important known methods of prevention is vector control, which has been effective only in certain areas of South America, the development of a vaccine to protect people at risk has been proposed. In this study, we assessed the cellular and humoral immune response generated following immunization with pBCSP and pBCSSP4 plasmids containing the genes encoding a trans-sialidase protein (present in all three forms of T. cruzi) and an amastigote specific glycoprotein, respectively, in a canine model. Thirty-five beagle dogs were divided randomly into 5 groups (n=7) and were immunized twice intramuscularly with 500 μg of pBCSSP4, pBCSP, pBk-CMV (empty plasmid) or saline solution. Fifteen days after the last immunization the 4 groups were infected intraperitoneally with 500,000 metacyclic trypomastigotes. The fifth group was unimmunized/infected. The parasitaemia in the immunized/infected dogs was for a shorter period (14 vs. 29 days) and the parasite load was lower. The concentration of IgG1 (0.612±0.019 O.D.) and IgG2 (1.167±0.097 O.D.) subclasses was measured (absorbance) 15 days after the last immunization with both recombinant plasmids, the majority of which were IgG2. The treatment of parasites using the serum from dogs immunized with pBCSP and pBCSSP4 plasmids produced 54% (±11.8) and 68% (±21.4) complement-mediated lysis, respectively. At 12 h post immunization, an increase in cytokines was not observed; however, vaccination with pBCSSP4 significantly increased the levels of IFN-γ and IL-10 at 9 months post-infection. The recombinant plasmid immunization stimulated the spleen cell proliferation showing a positive stimulatory index above 2.0. In conclusion, immunization using both genes effectively induces a humoral and cellular immune response.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 52 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 2%
Unknown 51 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 11 21%
Researcher 7 13%
Student > Doctoral Student 6 12%
Student > Master 5 10%
Other 4 8%
Other 8 15%
Unknown 11 21%
Readers by discipline Count As %
Agricultural and Biological Sciences 22 42%
Immunology and Microbiology 5 10%
Biochemistry, Genetics and Molecular Biology 3 6%
Veterinary Science and Veterinary Medicine 3 6%
Medicine and Dentistry 2 4%
Other 3 6%
Unknown 14 27%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 March 2013.
All research outputs
#3,548,395
of 4,507,211 outputs
Outputs from Veterinary Research
#345
of 417 outputs
Outputs of similar age
#70,056
of 89,268 outputs
Outputs of similar age from Veterinary Research
#11
of 12 outputs
Altmetric has tracked 4,507,211 research outputs across all sources so far. This one is in the 3rd percentile – i.e., 3% of other outputs scored the same or lower than it.
So far Altmetric has tracked 417 research outputs from this source. They receive a mean Attention Score of 2.7. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 89,268 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 12 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.