Title |
Efficient recovery of proteins from multiple source samples after trizol® or trizol®LS RNA extraction and long-term storage
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Published in |
BMC Genomics, March 2013
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DOI | 10.1186/1471-2164-14-181 |
Pubmed ID | |
Authors |
André ES Simões, Diane M Pereira, Joana D Amaral, Ana F Nunes, Sofia E Gomes, Pedro M Rodrigues, Adrian C Lo, Rudi D'Hooge, Clifford J Steer, Stephen N Thibodeau, Pedro M Borralho, Cecília MP Rodrigues |
Abstract |
Simultaneous isolation of nucleic acids and proteins from a single biological sample facilitates meaningful data interpretation and reduces time, cost and sampling errors. This is particularly relevant for rare human and animal specimens, often scarce, and/or irreplaceable. TRIzol(®) and TRIzol(®)LS are suitable for simultaneous isolation of RNA, DNA and proteins from the same biological sample. These reagents are widely used for RNA and/or DNA isolation, while reports on their use for protein extraction are limited, attributable to technical difficulties in protein solubilisation. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Spain | 1 | 50% |
France | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 50% |
Scientists | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
India | 4 | 1% |
Germany | 2 | <1% |
France | 1 | <1% |
Czechia | 1 | <1% |
United Kingdom | 1 | <1% |
Canada | 1 | <1% |
Spain | 1 | <1% |
United States | 1 | <1% |
Unknown | 256 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 56 | 21% |
Researcher | 42 | 16% |
Student > Master | 36 | 13% |
Student > Bachelor | 35 | 13% |
Student > Postgraduate | 16 | 6% |
Other | 38 | 14% |
Unknown | 45 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 72 | 27% |
Biochemistry, Genetics and Molecular Biology | 70 | 26% |
Medicine and Dentistry | 17 | 6% |
Neuroscience | 12 | 4% |
Pharmacology, Toxicology and Pharmaceutical Science | 11 | 4% |
Other | 36 | 13% |
Unknown | 50 | 19% |