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Efficient recovery of proteins from multiple source samples after trizol® or trizol®LS RNA extraction and long-term storage

Overview of attention for article published in BMC Genomics, March 2013
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Mentioned by

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2 X users

Citations

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106 Dimensions

Readers on

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266 Mendeley
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2 CiteULike
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Title
Efficient recovery of proteins from multiple source samples after trizol® or trizol®LS RNA extraction and long-term storage
Published in
BMC Genomics, March 2013
DOI 10.1186/1471-2164-14-181
Pubmed ID
Authors

André ES Simões, Diane M Pereira, Joana D Amaral, Ana F Nunes, Sofia E Gomes, Pedro M Rodrigues, Adrian C Lo, Rudi D'Hooge, Clifford J Steer, Stephen N Thibodeau, Pedro M Borralho, Cecília MP Rodrigues

Abstract

Simultaneous isolation of nucleic acids and proteins from a single biological sample facilitates meaningful data interpretation and reduces time, cost and sampling errors. This is particularly relevant for rare human and animal specimens, often scarce, and/or irreplaceable. TRIzol(®) and TRIzol(®)LS are suitable for simultaneous isolation of RNA, DNA and proteins from the same biological sample. These reagents are widely used for RNA and/or DNA isolation, while reports on their use for protein extraction are limited, attributable to technical difficulties in protein solubilisation.

X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 266 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
India 4 2%
Germany 2 <1%
France 1 <1%
Czechia 1 <1%
United Kingdom 1 <1%
Canada 1 <1%
Spain 1 <1%
United States 1 <1%
Unknown 254 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 56 21%
Researcher 41 15%
Student > Master 36 14%
Student > Bachelor 35 13%
Student > Postgraduate 16 6%
Other 36 14%
Unknown 46 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 72 27%
Biochemistry, Genetics and Molecular Biology 69 26%
Medicine and Dentistry 17 6%
Neuroscience 12 5%
Pharmacology, Toxicology and Pharmaceutical Science 11 4%
Other 34 13%
Unknown 51 19%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 March 2013.
All research outputs
#14,747,687
of 22,701,287 outputs
Outputs from BMC Genomics
#6,115
of 10,622 outputs
Outputs of similar age
#118,213
of 196,095 outputs
Outputs of similar age from BMC Genomics
#69
of 133 outputs
Altmetric has tracked 22,701,287 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 10,622 research outputs from this source. They receive a mean Attention Score of 4.7. This one is in the 37th percentile – i.e., 37% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 196,095 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 37th percentile – i.e., 37% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 133 others from the same source and published within six weeks on either side of this one. This one is in the 43rd percentile – i.e., 43% of its contemporaries scored the same or lower than it.